Randomly barcoded transposon mutant libraries for gut commensals II: Applying libraries for functional genetics.

Autor:	Voogdt CGP; Genome Biology Unit, EMBL Heidelberg, Meyerhofstraße 1, 69117 Heidelberg, Germany; Structural and Computational Biology Unit, EMBL, Heidelberg, Germany., Tripathi S; Department of Plant and Microbial Biology, University of California, Berkeley, Berkeley, CA 94720, USA., Bassler SO; Genome Biology Unit, EMBL Heidelberg, Meyerhofstraße 1, 69117 Heidelberg, Germany; Faculty of Biosciences, Heidelberg University, Grabengasse 1, 69117 Heidelberg, Germany., McKeithen-Mead SA; Department of Bioengineering, Stanford University, Stanford, CA 94305, USA; Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA., Guiberson ER; Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA., Koumoutsi A; Genome Biology Unit, EMBL Heidelberg, Meyerhofstraße 1, 69117 Heidelberg, Germany., Bravo AM; Department of Fundamental Microbiology, University of Lausanne, 1015 Lausanne, Switzerland., Buie C; Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA., Zimmermann M; Structural and Computational Biology Unit, EMBL, Heidelberg, Germany., Sonnenburg JL; Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA; Chan Zuckerberg Biohub, San Francisco, CA 94158, USA., Typas A; Genome Biology Unit, EMBL Heidelberg, Meyerhofstraße 1, 69117 Heidelberg, Germany; Structural and Computational Biology Unit, EMBL, Heidelberg, Germany. Electronic address: typas@embl.de., Deutschbauer AM; Department of Plant and Microbial Biology, University of California, Berkeley, Berkeley, CA 94720, USA; Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA. Electronic address: amdeutschbauer@lbl.gov., Shiver AL; Department of Bioengineering, Stanford University, Stanford, CA 94305, USA. Electronic address: ashiver@stanford.edu., Huang KC; Department of Bioengineering, Stanford University, Stanford, CA 94305, USA; Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA; Chan Zuckerberg Biohub, San Francisco, CA 94158, USA. Electronic address: kchuang@stanford.edu.
Jazyk:	angličtina
Zdroj:	Cell reports [Cell Rep] 2024 Jan 23; Vol. 43 (1), pp. 113519. Date of Electronic Publication: 2023 Dec 23.
DOI:	10.1016/j.celrep.2023.113519
Abstrakt:	The critical role of the intestinal microbiota in human health and disease is well recognized. Nevertheless, there are still large gaps in our understanding of the functions and mechanisms encoded in the genomes of most members of the gut microbiota. Genome-scale libraries of transposon mutants are a powerful tool to help us address this gap. Recent advances in barcoded transposon mutagenesis have dramatically lowered the cost of mutant fitness determination in hundreds of in vitro and in vivo experimental conditions. In an accompanying review, we discuss recent advances and caveats for the construction of pooled and arrayed barcoded transposon mutant libraries in human gut commensals. In this review, we discuss how these libraries can be used across a wide range of applications, the technical aspects involved, and expectations for such screens. Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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