| Autor: |
Guglielmotti V; Department of Chemistry, School of Analytical Sciences Adlershof (SALSA) & IRIS Adlershof, Humboldt-Universität zu Berlin, Berlin 10099, Germany.; Instituto de Nanosistemas, Universidad Nacional de General San Martín, San Martín 1650, Provincia de Buenos Aires, Argentina., Fuhry E; Department of Chemistry, School of Analytical Sciences Adlershof (SALSA) & IRIS Adlershof, Humboldt-Universität zu Berlin, Berlin 10099, Germany., Neubert TJ; Department of Chemistry, School of Analytical Sciences Adlershof (SALSA) & IRIS Adlershof, Humboldt-Universität zu Berlin, Berlin 10099, Germany., Kuhl M; Department of Chemistry, School of Analytical Sciences Adlershof (SALSA) & IRIS Adlershof, Humboldt-Universität zu Berlin, Berlin 10099, Germany., Pallarola D; Instituto de Nanosistemas, Universidad Nacional de General San Martín, San Martín 1650, Provincia de Buenos Aires, Argentina., Balasubramanian K; Department of Chemistry, School of Analytical Sciences Adlershof (SALSA) & IRIS Adlershof, Humboldt-Universität zu Berlin, Berlin 10099, Germany. |
| Abstrakt: |
Soft substrates are interesting for many applications, ranging from mimicking the cellular microenvironment to implants. Conductive electrodes on such substrates allow the realization of flexible, elastic, and transparent sensors. Single-layer graphene as a candidate for such electrodes brings the advantage that the active area of the sensor is transparent and conformal to the underlying substrate. Here, we overcome several challenges facing the routine realization of graphene cell sensors on a canonical soft substrate, namely, poly(dimethylsiloxane) (PDMS). We have systematically studied the effect of surface energy before, during, and after the transfer of graphene. Thus, we have identified a suitable support polymer, optimal substrate (pre)treatment, and an appropriate solvent for the removal of the support. Using this procedure, we can reproducibly obtain stable and intact graphene sensors on a millimeter scale on PDMS, which can withstand continuous measurements in cell culture media for several days. From local nanomechanical measurements, we infer that the softness of the substrate is slightly affected after the graphene transfer. However, we can modulate the stiffness using PDMS with differing compositions. Finally, we show that graphene sensors on PDMS can be successfully used as soft electrodes for real-time monitoring of the cell adhesion kinetics. The routine availability of single-layer graphene electrodes on a soft substrate with tunable stiffness will open a new avenue for studies, where the PDMS-liquid interface is made conducting with minimal alteration of the intrinsic material properties such as softness, flexibility, elasticity, and transparency. |
