A Novel Handrub Tablet Loaded with Pre- and Post-Biotic Solid Lipid Nanoparticles Combining Virucidal Activity and Maintenance of the Skin Barrier and Microbiome.

Autor: Machado ACHR; Programa de Pós-Graduação em Medicina Translacional, Universidade Federal de São Paulo, Rua Pedro de Toledo, 720, Sao Paulo 04039-002, SP, Brazil., Marinheiro LJ; Departamento de Ciências Farmacêuticas, Instituto de Ciências Ambientais, Químicas e Farmacêuticas, Universidade Federal de São Paulo, Rua São Nicolau, 210, Diadema 09913-030, SP, Brazil., Benson HAE; Curtin Medical School, Curtin University, Perth, WA 6845, Australia., Grice JE; Frazer Institute, Faculty of Medicine, The University of Queensland, Brisbane, QLD 4102, Australia., Martins TDS; Laboratório de Materiais Híbridos, Departamento de Química, Instituto de Ciências Ambientais, Químicas e Farmacêuticas, Universidade Federal de São Paulo, Rua São Nicolau, 210, 2° Andar, Diadema 09913-030, SP, Brazil., Lan A; Shanghai Pechoin Daily Chemical Corporation, Shanghai 200060, China., Lopes PS; Departamento de Ciências Farmacêuticas, Instituto de Ciências Ambientais, Químicas e Farmacêuticas, Universidade Federal de São Paulo, Rua São Nicolau, 210, Diadema 09913-030, SP, Brazil., Andreo-Filho N; Departamento de Ciências Farmacêuticas, Instituto de Ciências Ambientais, Químicas e Farmacêuticas, Universidade Federal de São Paulo, Rua São Nicolau, 210, Diadema 09913-030, SP, Brazil.; School of Pharmacy, The University of Queensland, Brisbane, QLD 4102, Australia., Leite-Silva VR; Programa de Pós-Graduação em Medicina Translacional, Universidade Federal de São Paulo, Rua Pedro de Toledo, 720, Sao Paulo 04039-002, SP, Brazil.; Departamento de Ciências Farmacêuticas, Instituto de Ciências Ambientais, Químicas e Farmacêuticas, Universidade Federal de São Paulo, Rua São Nicolau, 210, Diadema 09913-030, SP, Brazil.; Frazer Institute, Faculty of Medicine, The University of Queensland, Brisbane, QLD 4102, Australia.
Jazyk: angličtina
Zdroj: Pharmaceutics [Pharmaceutics] 2023 Dec 17; Vol. 15 (12). Date of Electronic Publication: 2023 Dec 17.
DOI: 10.3390/pharmaceutics15122793
Abstrakt: Objective: This study aimed to develop a holobiont tablet with rapid dispersibility to provide regulation of the microbiota, virucidal activity, and skin barrier protection.
Methods: A 2 3 factorial experiment was planned to define the best formulation for the development of the base tablet, using average weight, hardness, dimensions, swelling rate, and disintegration time as parameters to be analyzed. To produce holobiont tablets, the chosen base formulation was fabricated by direct compression of prebiotics, postbiotics, and excipients. The tablets also incorporated solid lipid nanoparticles containing postbiotics that were obtained by high-pressure homogenization and freeze-drying. The in vitro virucidal activity against alpha-coronavirus particles (CCoV-VR809) was determined in VERO cell culture. In vitro analysis, using monolayer cells and human equivalent skin, was performed by rRTq-PCR to determine the expression of interleukins 1, 6, 8, and 17, aquaporin-3, involucrin, filaggrin, FoxO3, and SIRT-1. Antioxidant activity and collagen-1 synthesis were also performed in fibroblast cells. Metagenomic analysis of the skin microbiome was determined in vivo before and after application of the holobiont tablet, during one week of continuous use, and compared to the use of alcohol gel. Samples were analyzed by sequencing the V3-V4 region of the 16S rRNA gene.
Results: A handrub tablet with rapid dispersibility was developed for topical use and rinse off. After being defined as safe, the virucidal activity was found to be equal to or greater than that of 70% alcohol, with a reduction in interleukins and maintenance or improvement of skin barrier gene markers, in addition to the reestablishment of the skin microbiota after use.
Conclusions: The holobiont tablets were able to improve the genetic markers related to the skin barrier and also its microbiota, thereby being more favorable for use as a hand sanitizer than 70% alcohol.
Databáze: MEDLINE
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