Formulation and Comprehensive Evaluation of Biohybrid Hydrogel Membranes Containing Doxycycline or Silver Nanoparticles.

Autor: Stan D; DDS Diagnostic, Segovia 1 Str., 031427 Bucharest, Romania.; Doctoral School of Medicine, Titu Maiorescu University, 040441 Bucharest, Romania., Ruta LL; DDS Diagnostic, Segovia 1 Str., 031427 Bucharest, Romania.; Department of Inorganic, Organic Chemistry, Biochemistry and Catalysis, Faculty of Chemistry, University of Bucharest, 90-92 Panduri Str., 050663 Bucharest, Romania., Bocancia-Mateescu LA; DDS Diagnostic, Segovia 1 Str., 031427 Bucharest, Romania., Mirica AC; DDS Diagnostic, Segovia 1 Str., 031427 Bucharest, Romania., Stan D; DDS Diagnostic, Segovia 1 Str., 031427 Bucharest, Romania., Micutz M; Department of Analytical and Physical Chemistry, University of Bucharest, 4-12 Regina Elisabeta Blvd., 030018 Bucharest, Romania., Brincoveanu O; National Institute for R&D in Microtechnology, 077190 Bucharest, Romania.; Research Institute, The University of Bucharest, 060102 Bucharest, Romania., Enciu AM; Victor Babes National Institute of Pathology, 050096 Bucharest, Romania.; Department of Cell Biology and Histology, Carol Davila University of Medicine and Pharmacy, 050474 Bucharest, Romania., Codrici E; Victor Babes National Institute of Pathology, 050096 Bucharest, Romania., Popescu ID; Victor Babes National Institute of Pathology, 050096 Bucharest, Romania., Popa ML; Department of Cell Biology and Histology, Carol Davila University of Medicine and Pharmacy, 050474 Bucharest, Romania., Rotaru F; Polytechnic University of Bucharest, Splaiul Independenței 54, 030167 Bucharest, Romania.; Rohealth-Health and Bioeconomy Cluster, Calea Griviţei 6-8, 010731 Bucharest, Romania.; Frontier Management Consulting, Calea Griviţei6-8, 010731 Bucharest, Romania., Tanase C; Victor Babes National Institute of Pathology, 050096 Bucharest, Romania.; Department of Cell Biology and Clinical Biochemistry, Titu Maiorescu University, 031593 Bucharest, Romania.
Jazyk: angličtina
Zdroj: Pharmaceutics [Pharmaceutics] 2023 Nov 28; Vol. 15 (12). Date of Electronic Publication: 2023 Nov 28.
DOI: 10.3390/pharmaceutics15122696
Abstrakt: Complicated wounds often require specialized medical treatments, and hydrogels have emerged as a popular choice for wound dressings in such cases due to their unique properties and the ability to incorporate and release therapeutic agents. Our focus was to develop and characterize a new optimized formula for biohybrid hydrogel membranes, which combine natural and synthetic polymers, bioactive natural compounds, like collagen and hyaluronic acid, and pharmacologically active substances (doxycycline or npAg). Dynamic (oscillatory) rheometry confirmed the strong gel-like properties of the obtained hydrogel membranes. Samples containing low-dose DOXY showed a swelling index of 285.68 ± 6.99%, a degradation rate of 71.6 ± 0.91% at 20 h, and achieved a cumulative drug release of approximately 90% at pH 7.4 and 80% at pH 8.3 within 12 h. The addition of npAg influenced the physical properties of the hydrogel membranes. Furthermore, the samples containing DOXY demonstrated exceptional antimicrobial efficacy against seven selected bacterial strains commonly associated with wound infections and complications. Biocompatibility assessments revealed that the samples exhibited over 80% cell viability. However, the addition of smaller-sized nanoparticles led to decreased cellular viability. The obtained biohybrid hydrogel membranes show favorable properties that render them suitable for application as wound dressings.
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje