| Autor: |
Antoshin A; Institute for Regenerative Medicine, Sechenov University, 8-2 Trubetskaya St., 119048 Moscow, Russia., Gostev M; Department of Oral Surgery, Borovskiy Institute of Dentistry, Sechenov University, 8-2 Trubetskaya St., 119048 Moscow, Russia., Khristidis Y; Institute for Regenerative Medicine, Sechenov University, 8-2 Trubetskaya St., 119048 Moscow, Russia., Giliazova A; Institute for Regenerative Medicine, Sechenov University, 8-2 Trubetskaya St., 119048 Moscow, Russia., Voloshin S; Institute for Regenerative Medicine, Sechenov University, 8-2 Trubetskaya St., 119048 Moscow, Russia., Blagushina N; Department of Oral Surgery, Borovskiy Institute of Dentistry, Sechenov University, 8-2 Trubetskaya St., 119048 Moscow, Russia., Smirnova O; Institute for Regenerative Medicine, Sechenov University, 8-2 Trubetskaya St., 119048 Moscow, Russia., Diachkova E; Department of Oral Surgery, Borovskiy Institute of Dentistry, Sechenov University, 8-2 Trubetskaya St., 119048 Moscow, Russia., Istranova E; Institute for Regenerative Medicine, Sechenov University, 8-2 Trubetskaya St., 119048 Moscow, Russia., Usanova A; Institute for Regenerative Medicine, Sechenov University, 8-2 Trubetskaya St., 119048 Moscow, Russia., Solodov N; Department of Oral Surgery, Borovskiy Institute of Dentistry, Sechenov University, 8-2 Trubetskaya St., 119048 Moscow, Russia., Fayzullin A; Institute for Regenerative Medicine, Sechenov University, 8-2 Trubetskaya St., 119048 Moscow, Russia., Ivanova E; Institute for Regenerative Medicine, Sechenov University, 8-2 Trubetskaya St., 119048 Moscow, Russia., Sadchikova E; Institute of Gene Biology, Russian Academy of Sciences, 34/5 Vavilov St., 119344 Moscow, Russia., Vergara Bashkatova MN; Department of Operative Surgery and Topographic Anatomy, Sechenov University, 8-2 Trubetskaya St., 119048 Moscow, Russia., Drakina O; Department of Operative Surgery and Topographic Anatomy, Sechenov University, 8-2 Trubetskaya St., 119048 Moscow, Russia., Tarasenko S; Department of Oral Surgery, Borovskiy Institute of Dentistry, Sechenov University, 8-2 Trubetskaya St., 119048 Moscow, Russia., Timashev P; Institute for Regenerative Medicine, Sechenov University, 8-2 Trubetskaya St., 119048 Moscow, Russia.; World-Class Research Center 'Digital Biodesign and Personalized Healthcare', Sechenov University, 8-2 Trubetskaya St., 119048 Moscow, Russia. |
| Abstrakt: |
The quality of soft tissue defect regeneration after dental surgeries largely determines their final success. Collagen membranes have been proposed for the healing of such defects, but in some cases, they do not guarantee a sufficient volume of the regenerated tissue and vascularization. For this purpose, lactoferrin, a protein with natural pro-regenerative, anti-inflammatory, and pro-angiogenic activity, can be added to collagen. In this article, we used a semipermeable barrier-assisted electrophoretic deposition (SBA-EPD) method for the production of collagen-lactoferrin membranes. The membrane structure was studied by SEM, and its mechanical properties were shown. The lactoferrin release kinetics were shown by ELISA within 75 h. When tested in vitro, we demonstrated that the collagen-lactoferrin membranes significantly increased the proliferation of keratinocytes (HaCaT) and fibroblasts (977hTERT) compared to blank collagen membranes. In vivo, on the vestibuloplasty and free gingival graft harvesting models, we showed that collagen-lactoferrin membranes decreased the wound inflammation and increased the healing rates and regeneration quality. In some parameters, collagen-lactoferrin membranes outperformed not only blank collagen membranes, but also the commercial membrane Mucograft ® . Thus, we proved that collagen-lactoferrin membranes produced by the SBA-EPD method may be a valuable alternative to commercially used membranes for soft tissue regeneration in the oral cavity. |
