| Autor: |
Chou HD; Department of Life Sciences, National Central University, Taoyuan 32001, Taiwan.; Department of Ophthalmology, Chang Gung Memorial Hospital, Linkou Main Branch, Taoyuan 33305, Taiwan.; College of Medicine, Chang Gung University, Taoyuan 33305, Taiwan.; National Institute of Cancer Research, National Health Research Institutes, Miaoli 35053, Taiwan., Shiah SG; Department of Life Sciences, National Central University, Taoyuan 32001, Taiwan.; National Institute of Cancer Research, National Health Research Institutes, Miaoli 35053, Taiwan., Chuang LH; College of Medicine, Chang Gung University, Taoyuan 33305, Taiwan.; Department of Ophthalmology, Chang Gung Memorial Hospital, Keelung 20401, Taiwan., Wu WC; Department of Ophthalmology, Chang Gung Memorial Hospital, Linkou Main Branch, Taoyuan 33305, Taiwan.; College of Medicine, Chang Gung University, Taoyuan 33305, Taiwan., Hwang YS; Department of Ophthalmology, Chang Gung Memorial Hospital, Linkou Main Branch, Taoyuan 33305, Taiwan.; College of Medicine, Chang Gung University, Taoyuan 33305, Taiwan., Chen KJ; Department of Ophthalmology, Chang Gung Memorial Hospital, Linkou Main Branch, Taoyuan 33305, Taiwan.; College of Medicine, Chang Gung University, Taoyuan 33305, Taiwan., Kang EY; Department of Ophthalmology, Chang Gung Memorial Hospital, Linkou Main Branch, Taoyuan 33305, Taiwan.; College of Medicine, Chang Gung University, Taoyuan 33305, Taiwan., Yeung L; College of Medicine, Chang Gung University, Taoyuan 33305, Taiwan.; Department of Ophthalmology, Chang Gung Memorial Hospital, Keelung 20401, Taiwan., Nien CY; Department of Life Sciences, National Central University, Taoyuan 32001, Taiwan., Lai CC; Department of Ophthalmology, Chang Gung Memorial Hospital, Linkou Main Branch, Taoyuan 33305, Taiwan.; College of Medicine, Chang Gung University, Taoyuan 33305, Taiwan.; Department of Ophthalmology, Chang Gung Memorial Hospital, Keelung 20401, Taiwan. |
| Abstrakt: |
Müller cells play a critical role in the closure of macular holes, and their proliferation and migration are facilitated by the internal limiting membrane (ILM). Despite the importance of this process, the underlying molecular mechanism remains underexplored. This study investigated the effects of ILM components on the microRNA (miRNA) profile of Müller cells. Rat Müller cells (rMC-1) were cultured with a culture insert and varying concentrations of ILM component coatings, namely, collagen IV, laminin, and fibronectin, and cell migration was assessed by measuring cell-free areas in successive photographs following insert removal. MiRNAs were then extracted from these cells and analyzed. Mimics and inhibitors of miRNA candidates were transfected into Müller cells, and a cell migration assay and additional cell viability assays were performed. The results revealed that the ILM components promoted Müller cell migration ( p < 0.01). Among the miRNA candidates, miR-194-3p was upregulated, whereas miR-125b-1-3p, miR-132-3p, miR-146b-5p, miR-152-3p, miR-196a-5p, miR-542-5p, miR-871-3p, miR-1839-5p, and miR-3573-3p were significantly downregulated ( p < 0.05; fold change > 1.5). Moreover, miR-152-3p and miR-196a-5p reduced cell migration ( p < 0.05) and proliferation ( p < 0.001), and their suppressive effects were reversed by their respective inhibitors. In conclusion, miRNAs were regulated in ILM component-activated Müller cells, with miR-152-3p and miR-196a-5p regulating Müller cell migration and proliferation. These results serve as a basis for understanding the molecular healing process of macular holes and identifying potential new target genes in future research. |
