| Autor: |
Olvera-Collantes L; Department of Biomedicine, Biotechnology and Public Health, School of Medicine, University of Cadiz, 11003 Cadiz, Spain.; Institute of Biomedical Research Cadiz (INIBICA), 11009 Cadiz, Spain., Moares N; Department of Biomedicine, Biotechnology and Public Health, School of Medicine, University of Cadiz, 11003 Cadiz, Spain., Fernandez-Cisnal R; Department of Biomedicine, Biotechnology and Public Health, School of Medicine, University of Cadiz, 11003 Cadiz, Spain.; Institute of Biomedical Research Cadiz (INIBICA), 11009 Cadiz, Spain., Muñoz-Miranda JP; Department of Biomedicine, Biotechnology and Public Health, School of Medicine, University of Cadiz, 11003 Cadiz, Spain.; Institute of Biomedical Research Cadiz (INIBICA), 11009 Cadiz, Spain., Gonzalez-Garcia P; Institute of Biomedical Research Cadiz (INIBICA), 11009 Cadiz, Spain., Gabucio A; Department of Biomedicine, Biotechnology and Public Health, School of Medicine, University of Cadiz, 11003 Cadiz, Spain., Freyre-Carrillo C; Microbiology Service, Puerto Real University Hospital, 11510 Puerto Real, Spain., Jordan-Chaves JD; Microbiology Service, Puerto Real University Hospital, 11510 Puerto Real, Spain., Trujillo-Soto T; Microbiology Service, Puerta del Mar University Hospital, 11009 Cadiz, Spain., Rodriguez-Martinez MP; La Milagrosa Health Centre, 11406 Jerez de la Frontera, Spain., Martin-Rubio MI; Jerez Costa Noroeste Health District, 11403 Jerez de la Frontera, Spain., Escuer E; Jerez University Hospital, 11407 Jerez de la Frontera, Spain., Rodriguez-Iglesias M; Department of Biomedicine, Biotechnology and Public Health, School of Medicine, University of Cadiz, 11003 Cadiz, Spain.; Institute of Biomedical Research Cadiz (INIBICA), 11009 Cadiz, Spain.; Microbiology Service, Puerta del Mar University Hospital, 11009 Cadiz, Spain., Fernandez-Ponce C; Department of Biomedicine, Biotechnology and Public Health, School of Medicine, University of Cadiz, 11003 Cadiz, Spain.; Institute of Biomedical Research Cadiz (INIBICA), 11009 Cadiz, Spain., Garcia-Cozar F; Department of Biomedicine, Biotechnology and Public Health, School of Medicine, University of Cadiz, 11003 Cadiz, Spain.; Institute of Biomedical Research Cadiz (INIBICA), 11009 Cadiz, Spain. |
| Abstrakt: |
There is still a long way ahead regarding the COVID-19 pandemic, since emerging waves remain a daunting challenge to the healthcare system. For this reason, the development of new preventive tools and therapeutic strategies to deal with the disease have been necessary, among which serological assays have played a key role in the control of COVID-19 outbreaks and vaccine development. Here, we have developed and evaluated an immunoassay capable of simultaneously detecting multiple IgG antibodies against different SARS-CoV-2 antigens through the use of Bio-Plex TM technology. Additionally, we have analyzed the antibody response in COVID-19 patients with different clinical profiles in Cadiz, Spain. The multiplex immunoassay presented is a high-throughput and robust immune response monitoring tool capable of concurrently detecting anti-S1, anti-NC and anti-RBD IgG antibodies in serum with a very high sensitivity (94.34-97.96%) and specificity (91.84-100%). Therefore, the immunoassay proposed herein may be a useful monitoring tool for individual humoral immunity against SARS-CoV-2, as well as for epidemiological surveillance. In addition, we show the values of antibodies against multiple SARS-CoV-2 antigens and their correlation with the different clinical profiles of unvaccinated COVID-19 patients in Cadiz, Spain, during the first and second waves of the pandemic. |
