| Autor: |
Poenariu IS; Doctoral School, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania.; Department of Immunology, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania., Boldeanu L; Department of Microbiology, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania., Ungureanu BS; Department of Gastroenterology, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania., Caragea DC; Department of Nephrology, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania., Cristea OM; Department of Microbiology, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania., Pădureanu V; Department of Internal Medicine, Faculty of Medicine, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania., Siloși I; Department of Immunology, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania., Ungureanu AM; Department of Microbiology, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania., Statie RC; Doctoral School, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania.; Department of Gastroenterology, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania., Ciobanu AE; Doctoral School, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania., Gheonea DI; Department of Gastroenterology, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania., Osiac E; Department of Biophysics, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania., Boldeanu MV; Department of Immunology, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania.; Medico Science SRL-Stem Cell Bank Unit, 200690 Craiova, Romania. |
| Abstrakt: |
We intended to investigate the presence and medical application of serum hypoxia-inducible factor-1 alpha (HIF-1α) along with the already known systemic inflammatory markers and the new one's inflammatory indices, the proportion of mean corpuscular volume and lymphocytes (MCVL) and the cumulative inflammatory index (IIC), for patients with ulcerative colitis (UC). We sought to establish correlations that may be present between the serum levels of HIF-1α and these inflammatory indices, as well as their relationship with disease activity and the extent of UC, which can provide us with a more precise understanding of the evolution, prognosis, and future well-being of patients. Serum samples were collected from 46 patients diagnosed with UC and 23 controls. For our assessment of the serum levels of HIF-1α, we used the Enzyme-Linked Immunosorbent Assay (ELISA) technique. Thus, for HIF-1α we detected significantly higher values in more severe and more extensive UC. When it came to MCVL and IIC, we observed statistically significant differences between the three groups being compared (Severe, Moderate, and Mild). Our study highlighted that HIF-1α correlated much better with a disease activity score, MCVL, and IIC. With MCVL and IIC, a strong and very strong correlation had formed between them and well-known inflammation indices. By examining the ROC curves of the analyzed parameters, we recognized that TWI (accuracy of 83.70%) provides the best discrimination of patients with early forms of UC, followed by HIF-1α (73.90% accuracy), MCVL (70.90% accuracy), and PLR (70.40%). In our study, we observed that HIF-1α, MCVL, and PLR had the same sensitivity (73.33%) but HIF-1α had a much better specificity (60.87% vs. 58.70%, and 54.35%). Also, in addition to the PLR, HIF-1α and MCVL can be used as independent predictor factors in the discrimination of patients with early forms of UC. |
