Protective Properties of S-layer Protein 2 from Lactobacillus crispatus 2029 against Candida albicans Infections.

Autor: Abramov VM; Federal Service for Veterinary and Phytosanitary Surveillance (Rosselkhoznadzor) Federal State Budgetary Institution 'The Russian State Center for Animal Feed and Drug Standardization and Quality' (FGBU VGNKI), 123022 Moscow, Russia.; Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health, 117997 Moscow, Russia., Kosarev IV; Federal Service for Veterinary and Phytosanitary Surveillance (Rosselkhoznadzor) Federal State Budgetary Institution 'The Russian State Center for Animal Feed and Drug Standardization and Quality' (FGBU VGNKI), 123022 Moscow, Russia.; Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health, 117997 Moscow, Russia., Machulin AV; Skryabin Institute of Biochemistry and Physiology of Microorganisms, Federal Research Center 'Pushchino Scientific Center for Biological Research of Russian Academy of Science', Russian Academy of Science, 142290 Pushchino, Russia., Priputnevich TV; Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health, 117997 Moscow, Russia., Deryusheva EI; Institute for Biological Instrumentation, Federal Research Center 'Pushchino Scientific Center for Biological Research of Russian Academy of Science', Russian Academy of Science, 142290 Pushchino, Russia., Panin AN; Federal Service for Veterinary and Phytosanitary Surveillance (Rosselkhoznadzor) Federal State Budgetary Institution 'The Russian State Center for Animal Feed and Drug Standardization and Quality' (FGBU VGNKI), 123022 Moscow, Russia., Chikileva IO; Laboratory of Cell Immunity, Blokhin National Research Center of Oncology, Ministry of Health RF, 115478 Moscow, Russia., Abashina TN; Skryabin Institute of Biochemistry and Physiology of Microorganisms, Federal Research Center 'Pushchino Scientific Center for Biological Research of Russian Academy of Science', Russian Academy of Science, 142290 Pushchino, Russia., Melnikov VG; Gabrichevsky Research Institute for Epidemiology and Microbiology, 125212 Moscow, Russia., Suzina NE; Skryabin Institute of Biochemistry and Physiology of Microorganisms, Federal Research Center 'Pushchino Scientific Center for Biological Research of Russian Academy of Science', Russian Academy of Science, 142290 Pushchino, Russia., Nikonov IN; Federal State Educational Institution of Higher Professional Education Moscow State Academy of Veterinary Medicine and Biotechnology Named after K.I. Skryabin, 109472 Moscow, Russia., Akhmetzyanova AA; Federal Service for Veterinary and Phytosanitary Surveillance (Rosselkhoznadzor) Federal State Budgetary Institution 'The Russian State Center for Animal Feed and Drug Standardization and Quality' (FGBU VGNKI), 123022 Moscow, Russia., Khlebnikov VS; Institute of Immunological Engineering, 142380 Lyubuchany, Russia., Sakulin VK; Institute of Immunological Engineering, 142380 Lyubuchany, Russia., Vasilenko RN; Institute of Immunological Engineering, 142380 Lyubuchany, Russia., Samoilenko VA; Skryabin Institute of Biochemistry and Physiology of Microorganisms, Federal Research Center 'Pushchino Scientific Center for Biological Research of Russian Academy of Science', Russian Academy of Science, 142290 Pushchino, Russia., Gordeev AB; Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health, 117997 Moscow, Russia., Sukhikh GT; Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health, 117997 Moscow, Russia., Uversky VN; Department of Molecular Medicine, Morsani College of Medicine, University of South Florida, Tampa, FL 33612, USA., Karlyshev AV; Department of Biomolecular Sciences, School of Life Sciences, Chemistry and Pharmacy, Faculty of Health, Science, Social Care and Education, Kingston University London, Kingston upon Thames KT1 2EE, UK.
Jazyk: angličtina
Zdroj: Biomolecules [Biomolecules] 2023 Dec 04; Vol. 13 (12). Date of Electronic Publication: 2023 Dec 04.
DOI: 10.3390/biom13121740
Abstrakt: Previously, the protective role of the S-layer protein 2 (Slp2) of the vaginal Lactobacillus crispatus 2029 (LC2029) strain against foodborne pathogens Campylobacter jejuni , Salmonella enterica serovar Enteritidis, and Escherichia coli O157:H was demonstrated. We demonstrate the new roles of the Slp2-positive LC2029 strain and soluble Slp2 against C. albicans infections. We show that LC2029 bacteria can adhere to the surface of the cervical epithelial HeLa cells, prevent their contact with C. albicans , and block yeast transition to a pathogenic hyphal form. Surface-bound Slp2 provides the ability for LC2029 to co-aggregate with various C. albicans strains, including clinical isolates. C. albicans -induced necrotizing epithelial damage is reduced by colonization with the Slp2-positive LC2029 strain. Slp2 inhibits the adhesion of various strains of C. albicans to different human epithelial cells, blocks yeast transition to a pathogenic hyphal form, and prevents the colonization and pathogenic infiltration of mucosal barriers. Only Slp2 and LC2029 bacteria stimulate the production of protective human β-defensin 3 in various epithelial cells. These findings support the anti- Candida albicans potential of the probiotic LC2029 strain and Slp2 and form the basis for further research on their ability to prevent and manage invasive Candida infections.
Databáze: MEDLINE
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