Neutrophils are involved in early bone formation during midpalatal expansion.

Autor: Jiang T; Department of Orthodontics, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.; College of Stomatology, Shanghai Jiao Tong University, Shanghai, China.; National Center for Stomatology, Shanghai, China.; National Clinical Research Center for Oral Diseases, Shanghai, China.; Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, Shanghai, China.; Research Unit of Oral and Maxillofacial Regenerative Medicine, Chinese Academy of Medical Sciences, Shanghai, China., Tang XY; Department of Orthodontics, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.; College of Stomatology, Shanghai Jiao Tong University, Shanghai, China.; National Center for Stomatology, Shanghai, China.; National Clinical Research Center for Oral Diseases, Shanghai, China.; Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, Shanghai, China.; Research Unit of Oral and Maxillofacial Regenerative Medicine, Chinese Academy of Medical Sciences, Shanghai, China., Su H; Department of Orthodontics, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.; College of Stomatology, Shanghai Jiao Tong University, Shanghai, China.; National Center for Stomatology, Shanghai, China.; National Clinical Research Center for Oral Diseases, Shanghai, China.; Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, Shanghai, China.; Research Unit of Oral and Maxillofacial Regenerative Medicine, Chinese Academy of Medical Sciences, Shanghai, China., Chen JY; Department of Orthodontics, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.; College of Stomatology, Shanghai Jiao Tong University, Shanghai, China.; National Center for Stomatology, Shanghai, China.; National Clinical Research Center for Oral Diseases, Shanghai, China.; Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, Shanghai, China.; Research Unit of Oral and Maxillofacial Regenerative Medicine, Chinese Academy of Medical Sciences, Shanghai, China., Qin YQ; Department of Orthodontics, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.; College of Stomatology, Shanghai Jiao Tong University, Shanghai, China.; National Center for Stomatology, Shanghai, China.; National Clinical Research Center for Oral Diseases, Shanghai, China.; Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, Shanghai, China.; Research Unit of Oral and Maxillofacial Regenerative Medicine, Chinese Academy of Medical Sciences, Shanghai, China., Qin YC; Department of Orthodontics, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.; College of Stomatology, Shanghai Jiao Tong University, Shanghai, China.; National Center for Stomatology, Shanghai, China.; National Clinical Research Center for Oral Diseases, Shanghai, China.; Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, Shanghai, China.; Research Unit of Oral and Maxillofacial Regenerative Medicine, Chinese Academy of Medical Sciences, Shanghai, China., Ouyang NJ; Department of Orthodontics, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.; College of Stomatology, Shanghai Jiao Tong University, Shanghai, China.; National Center for Stomatology, Shanghai, China.; National Clinical Research Center for Oral Diseases, Shanghai, China.; Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, Shanghai, China., Tang GH; Department of Orthodontics, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.; College of Stomatology, Shanghai Jiao Tong University, Shanghai, China.; National Center for Stomatology, Shanghai, China.; National Clinical Research Center for Oral Diseases, Shanghai, China.; Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, Shanghai, China.; Research Unit of Oral and Maxillofacial Regenerative Medicine, Chinese Academy of Medical Sciences, Shanghai, China.
Jazyk: angličtina
Zdroj: Oral diseases [Oral Dis] 2024 Oct; Vol. 30 (7), pp. 4483-4494. Date of Electronic Publication: 2023 Dec 22.
DOI: 10.1111/odi.14849
Abstrakt: Objective: Midpalatal expansion (MPE) is routinely employed to treat transverse maxillary arch deficiency. Neutrophils are indispensable for recruiting bone marrow stromal cells (BMSCs) at the initial stage of bone regeneration. This study aimed to explore whether neutrophils participate in MPE and how they function during bone formation under mechanical stretching.
Materials and Methods: The presence and phenotype of neutrophils in the midpalatal suture during expansion were detected by flow cytometry and immunofluorescence staining. The possible mechanism of neutrophil recruitment and polarization was explored in vitro by exposing vascular endothelial cells (VECs) to cyclic tensile strain.
Results: The number of neutrophils in the distracted suture peaked on Day 3, and N2-type neutrophils significantly increased on Day 5 after force application. The depletion of circulatory neutrophils reduced bone volume by 43.6% after 7-day expansion. The stretched VECs recruited neutrophils via a CXCR2 mechanism in vitro, which then promoted BMSC osteogenic differentiation through the VEGFA/VEGFR2 axis. Consistently, these neutrophils showed higher expression of canonical N2 phenotype genes, including CD206 and Arg1.
Conclusions: These results suggested that neutrophils participated in early bone formation during MPE. Based on these findings, we propose that stretched VECs recruited and polarized neutrophils, which, in turn, induced BMSC osteogenic differentiation.
(© 2023 Wiley Periodicals LLC.)
Databáze: MEDLINE
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