Detection of a new avian bornavirus in barn owl (Tyto alba) by pan-viral microarray.

Autor: Aguilera-Sepúlveda P; Centro de Investigación en Sanidad Animal (CISA-INIA), CSIC, 28130 Valdeolmos, Spain., Llorente F; Centro de Investigación en Sanidad Animal (CISA-INIA), CSIC, 28130 Valdeolmos, Spain., Rosenstierne MW; QlifeAps, Industriparken 39-41, DK-2750 Ballerup, Denmark; Virus Research & Development Laboratory Statens Serum Institut, Copenhagen, Denmark., Bravo-Barriga D; Departamento de Sanidad Animal, Grupo de Investigación en Salud Animal y Zoonosis (GISAZ), UIC Zoonosis y Enfermedades Emergentes (ENZOEM), Facultad de Veterinaria, Universidad de Córdoba, Córdoba, Spain., Frontera E; Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad de Extremadura, Cáceres, Spain., Fomsgaard A; Virus Research & Development Laboratory Statens Serum Institut, Copenhagen, Denmark., Fernández-Pinero J; Centro de Investigación en Sanidad Animal (CISA-INIA), CSIC, 28130 Valdeolmos, Spain., Jiménez-Clavero MÁ; Centro de Investigación en Sanidad Animal (CISA-INIA), CSIC, 28130 Valdeolmos, Spain; CIBER of Epidemiology and Public Health (CIBERESP), 28029 Madrid, Spain. Electronic address: majimenez@inia.csic.es.
Jazyk: angličtina
Zdroj: Veterinary microbiology [Vet Microbiol] 2024 Feb; Vol. 289, pp. 109959. Date of Electronic Publication: 2023 Dec 19.
DOI: 10.1016/j.vetmic.2023.109959
Abstrakt: A barn owl (Tyto alba) died with neurological signs compatible with a viral infection. After discarding other possible infections caused by circulating viruses in the area, analysis of the central nervous system using a pan-viral microarray revealed hybridization to canary bornavirus 2 (CnBV-2). Subsequent sequence analysis confirmed the presence of a virus sharing more than 83% identity with CnBV-2. Surprisingly, the new sequence corresponds to a new virus, here named Barn owl Bornavirus 1 (BoBV-1), within the Orthobornavirus serini species. Moreover, it is the first member of this species that has been detected in a non-passerine bird, indicating that Orthobornavirus serini species comprises viruses with a wider range of hosts than previously presumed. The use of this microarray has proven to be an excellent tool for viral detection in clinical samples, with capacity to detect new viral variants. This allows the diagnosis of a great range of viruses, which can cause similar disease symptoms and which identification by PCR methods might be tedious, probably unsuccessful and, in the long run, expensive. This platform is highly useful for a fast and precise viral detection, contributing to the improvement of diagnostic methods.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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