Testicular piRNA Analysis Identified Dysregulated piRNAs in Non-obstructive Azoospermia.

Autor: Piryaei F; Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran. f.piryaei@umsha.ac.ir., Mehta P; Male Reproductive Biology Laboratory, Central Drug Research Institute, Lucknow, India.; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, India., Mozdarani H; Department of Medical Genetics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran., Hamzehlooy F; Department of Medical Biotechnology, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran., Barati M; Department of Medical Biotechnology, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran.; Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran., Piryaei Z; Department of Bioinformatics, Kish International Campus, University of Tehran, Kish, Iran., Gilani MAS; Department of Andrology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran., Alemi M; Urology and Nephrology Research Center, Hamadan University of Medical Sciences, Hamadan, Iran., Singh R; Male Reproductive Biology Laboratory, Central Drug Research Institute, Lucknow, India.; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, India.
Jazyk: angličtina
Zdroj: Reproductive sciences (Thousand Oaks, Calif.) [Reprod Sci] 2024 May; Vol. 31 (5), pp. 1246-1255. Date of Electronic Publication: 2023 Dec 22.
DOI: 10.1007/s43032-023-01433-3
Abstrakt: Male infertility has remained idiopathic in a remarkable proportion of all cases. Gonadal expression of PIWI-interacting RNAs (piRNAs) has been shown to be vital to normal spermatogenesis, as they are expressed in almost all types of testicular germ cells. These molecules and their related Piwi proteins strictly regulate transposable elements' activity and gene expression. We aimed to identify dysregulated piRNAs in idiopathic non-obstructive azoospermic (NOA) testis by global expression analysis. Testis tissue samples from 18 azoospermic patients (ten NOA and eight OA) were studied by small RNA sequencing. To validate high-throughput sequencing data, quantitative real-time polymerase chain reactions for two differentially altered piRNAs were performed. Bioinformatics analyses were undertaken to identify pathways affected by piRNA dysregulation. In the NOA group, 1328 piRNAs were identified to be differentially expressed, of which 1322 were downregulated and 6 were upregulated. Bioinformatics analysis corroborated the involvement of dysregulated piRNA in spermatogenesis. We also identified 64 clusters of differentially expressed piRNAs, of which 42 clusters had a minimum of ten absolute piRNA hits. Our study suggests that piRNAs show significant dysregulation in infertility. Their target genes play a role in their self-biogenesis, probably by regulating their own production through a feedback mechanism. The downregulated piRNAs may find value as biomarkers for the presence of spermatozoa in the testis of azoospermic individuals, while the upregulated piRNAs are great candidates for further investigation of their precise functions in spermatogenesis.
(© 2023. The Author(s), under exclusive licence to Society for Reproductive Investigation.)
Databáze: MEDLINE
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