| Autor: |
Geka G; Immunoassays/Immunosensors Lab, Institute of Nuclear & Radiological Sciences & Technology, Energy & Safety, NCSR 'Demokritos', 15341 Aghia Paraskevi, Greece.; Department of Chemistry, National and Kapodistrian, University of Athens, University Campus, 15771 Athens, Greece., Kanioura A; Immunoassays/Immunosensors Lab, Institute of Nuclear & Radiological Sciences & Technology, Energy & Safety, NCSR 'Demokritos', 15341 Aghia Paraskevi, Greece., Kochylas I; Section of Condensed Matter Physics, Department of Physics, National and Kapodistrian University of Athens, University Campus, 15784 Athens, Greece., Likodimos V; Section of Condensed Matter Physics, Department of Physics, National and Kapodistrian University of Athens, University Campus, 15784 Athens, Greece., Gardelis S; Section of Condensed Matter Physics, Department of Physics, National and Kapodistrian University of Athens, University Campus, 15784 Athens, Greece., Dimitriou A; Institute of Nanoscience & Nanotechnology, NCSR 'Demokritos', 15341 Aghia Paraskevi, Greece., Papanikolaou N; Institute of Nanoscience & Nanotechnology, NCSR 'Demokritos', 15341 Aghia Paraskevi, Greece., Chatzantonaki K; Molecular Diagnosis Department, INVITROLABS S.A., 12251 Peristeri, Greece., Charvalos E; Molecular Diagnosis Department, INVITROLABS S.A., 12251 Peristeri, Greece., Economou A; Department of Chemistry, National and Kapodistrian, University of Athens, University Campus, 15771 Athens, Greece., Kakabakos S; Immunoassays/Immunosensors Lab, Institute of Nuclear & Radiological Sciences & Technology, Energy & Safety, NCSR 'Demokritos', 15341 Aghia Paraskevi, Greece., Petrou P; Immunoassays/Immunosensors Lab, Institute of Nuclear & Radiological Sciences & Technology, Energy & Safety, NCSR 'Demokritos', 15341 Aghia Paraskevi, Greece. |
| Abstrakt: |
Nanostructured noble metal surfaces enhance the photoluminescence emitted by fluorescent molecules, permitting the development of highly sensitive fluorescence immunoassays. To this end, surfaces with silicon nanowires decorated with silver nanoparticles in the form of dendrites or aggregates were evaluated as substrates for the immunochemical detection of two ovarian cancer indicators, carbohydrate antigen 125 (CA125) and human epididymis protein 4 (HE4). The substrates were prepared by metal-enhanced chemical etching of silicon wafers to create, in one step, silicon nanowires and silver nanoparticles on top of them. For both analytes, non-competitive immunoassays were developed using pairs of highly specific monoclonal antibodies, one for analyte capture on the substrate and the other for detection. In order to facilitate the identification of the immunocomplexes through a reaction with streptavidin labeled with Rhodamine Red-X, the detection antibodies were biotinylated. An in-house-developed optical set-up was used for photoluminescence signal measurements after assay completion. The detection limits achieved were 2.5 U/mL and 3.12 pM for CA125 and HE4, respectively, with linear dynamic ranges extending up to 500 U/mL for CA125 and up to 500 pM for HE4, covering the concentration ranges of both healthy and ovarian cancer patients. Thus, the proposed method could be implemented for the early diagnosis and/or prognosis and monitoring of ovarian cancer. |
