A Synergic Strategy: Adipose-Derived Stem Cell Spheroids Seeded on 3D-Printed PLA/CHA Scaffolds Implanted in a Bone Critical-Size Defect Model.

Autor:	Kronemberger GS; Nucleus of Multidisciplinary Research in Biology (Numpex-Bio), Federal University of Rio de Janeiro (UFRJ) Xerém, Duque de Caxias 25245-390, RJ, Brazil.; Laboratory of Eukariotic Cells, National Institute of Metrology, Quality and Technology (Inmetro), Duque de Caxias 25250-020, RJ, Brazil.; Post-Graduation Program of Translational Biomedicine (Biotrans), Unigranrio, Campus I, Duque de Caxias 25071-202, RJ, Brazil., Palhares TN; Brazilian Center for Physics Research, Xavier Sigaud 150, Urca 22290-180, RJ, Brazil., Rossi AM; Brazilian Center for Physics Research, Xavier Sigaud 150, Urca 22290-180, RJ, Brazil., Verçosa BRF; Nucleus of Multidisciplinary Research in Biology (Numpex-Bio), Federal University of Rio de Janeiro (UFRJ) Xerém, Duque de Caxias 25245-390, RJ, Brazil., Sartoretto SC; Laboratory of Clinical Research in Odontology, Fluminense Federal University (UFF), Niterói 24020-140, RJ, Brazil., Resende R; Laboratory of Clinical Research in Odontology, Fluminense Federal University (UFF), Niterói 24020-140, RJ, Brazil., Uzeda MJ; Laboratory of Clinical Research in Odontology, Fluminense Federal University (UFF), Niterói 24020-140, RJ, Brazil., Alves ATNN; Laboratory of Clinical Research in Odontology, Fluminense Federal University (UFF), Niterói 24020-140, RJ, Brazil., Alves GG; Laboratory of Clinical Research in Odontology, Fluminense Federal University (UFF), Niterói 24020-140, RJ, Brazil., Calasans-Maia MD; Laboratory of Clinical Research in Odontology, Fluminense Federal University (UFF), Niterói 24020-140, RJ, Brazil., Granjeiro JM; Laboratory of Eukariotic Cells, National Institute of Metrology, Quality and Technology (Inmetro), Duque de Caxias 25250-020, RJ, Brazil.; Post-Graduation Program of Translational Biomedicine (Biotrans), Unigranrio, Campus I, Duque de Caxias 25071-202, RJ, Brazil.; Laboratory of Clinical Research in Odontology, Fluminense Federal University (UFF), Niterói 24020-140, RJ, Brazil., Baptista LS; Nucleus of Multidisciplinary Research in Biology (Numpex-Bio), Federal University of Rio de Janeiro (UFRJ) Xerém, Duque de Caxias 25245-390, RJ, Brazil.; Laboratory of Eukariotic Cells, National Institute of Metrology, Quality and Technology (Inmetro), Duque de Caxias 25250-020, RJ, Brazil.; Post-Graduation Program of Translational Biomedicine (Biotrans), Unigranrio, Campus I, Duque de Caxias 25071-202, RJ, Brazil.
Jazyk:	angličtina
Zdroj:	Journal of functional biomaterials [J Funct Biomater] 2023 Nov 21; Vol. 14 (12). Date of Electronic Publication: 2023 Nov 21.
DOI:	10.3390/jfb14120555
Abstrakt:	Bone critical-size defects and non-union fractures have no intrinsic capacity for self-healing. In this context, the emergence of bone engineering has allowed the development of functional alternatives. The aim of this study was to evaluate the capacity of ASC spheroids in bone regeneration using a synergic strategy with 3D-printed scaffolds made from poly (lactic acid) (PLA) and nanostructured hydroxyapatite doped with carbonate ions (CHA) in a rat model of cranial critical-size defect. In summary, a set of results suggests that ASC spheroidal constructs promoted bone regeneration. In vitro results showed that ASC spheroids were able to spread and interact with the 3D-printed scaffold, synthesizing crucial growth factors and cytokines for bone regeneration, such as VEGF. Histological results after 3 and 6 months of implantation showed the formation of new bone tissue in the PLA/CHA scaffolds that were seeded with ASC spheroids. In conclusion, the presence of ASC spheroids in the PLA/CHA 3D-printed scaffolds seems to successfully promote bone formation, which can be crucial for a significant clinical improvement in critical bone defect regeneration.
Databáze:	MEDLINE
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