| Autor: |
Shchulkin AV; Department of Pharmacology, Ryazan State Medical University, 390026 Ryazan, Russia., Abalenikhina YV; Department of Pharmacology, Ryazan State Medical University, 390026 Ryazan, Russia., Slepnev AA; Department of Pharmacology, Ryazan State Medical University, 390026 Ryazan, Russia., Rokunov ED; Department of Pharmacology, Ryazan State Medical University, 390026 Ryazan, Russia., Yakusheva EN; Department of Pharmacology, Ryazan State Medical University, 390026 Ryazan, Russia. |
| Jazyk: |
angličtina |
| Zdroj: |
Current issues in molecular biology [Curr Issues Mol Biol] 2023 Nov 29; Vol. 45 (12), pp. 9593-9605. Date of Electronic Publication: 2023 Nov 29. |
| DOI: |
10.3390/cimb45120600 |
| Abstrakt: |
Organic anion transporting polypeptide 1B1 (OATP1B1) is an influx transporter protein of the SLC superfamily, expressed mainly in the liver and some tumor cells. The mechanisms of its regulation are being actively studied. In the present study, the effect of sex hormones (estradiol, progesterone and testosterone) on OATP1B1 expression in HepG2 cells was examined. The role of adopted orphan receptors, farnasoid X receptor (FXR), constitutive androstane receptor (CAR), pregnane X receptor (PXR) and liver X receptor subtype alpha (LXR a ), was also evaluated. Hormones were used in concentrations of 1, 10 and 100 μM, with incubation for 24 h. The protein expression of OATP1B1, FXR, CAR, PXR and LXR a was analyzed by Western blot. It was shown that estradiol (10 and 100 μM) increased the expression of OATP1B1, acting through CAR. Testosterone (1, 10 and 100 μM) increased the expression of OATP1B1, acting through FXR, PXR and LXR a . Progesterone (10 and 100 μM) decreased the expression of OATP1B1 (10 and 100 μM) and adopted orphan receptors are not involved in this process. The obtained results have important practical significance and determine ways for targeted regulation of the transporter, in particular in cancer. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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