| Autor: |
Dinić J; Department of Neurobiology, Institute for Biological Research 'Siniša Stanković'-National Institute of the Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11108 Belgrade, Serbia., Podolski-Renić A; Department of Neurobiology, Institute for Biological Research 'Siniša Stanković'-National Institute of the Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11108 Belgrade, Serbia., Dragoj M; Department of Neurobiology, Institute for Biological Research 'Siniša Stanković'-National Institute of the Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11108 Belgrade, Serbia., Jovanović Stojanov S; Department of Neurobiology, Institute for Biological Research 'Siniša Stanković'-National Institute of the Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11108 Belgrade, Serbia., Stepanović A; Department of Neurobiology, Institute for Biological Research 'Siniša Stanković'-National Institute of the Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11108 Belgrade, Serbia., Lupšić E; Department of Neurobiology, Institute for Biological Research 'Siniša Stanković'-National Institute of the Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11108 Belgrade, Serbia., Pajović M; Department of Neurobiology, Institute for Biological Research 'Siniša Stanković'-National Institute of the Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11108 Belgrade, Serbia., Jovanović M; Department of Neurobiology, Institute for Biological Research 'Siniša Stanković'-National Institute of the Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11108 Belgrade, Serbia., Petrović Rodić D; Department of Thoracic Pathology, Clinical Center of Serbia, Service of Pathohistology, University of Belgrade, Pasterova 2, 11000 Belgrade, Serbia., Marić D; Clinic for Pulmonology, Faculty of Medicine, University of Belgrade, Dr Koste Todorovića 26, 11000 Belgrade, Serbia., Ercegovac M; Clinic for Thoracic Surgery, Faculty of Medicine, University of Belgrade, Pasterova 2, 11000 Belgrade, Serbia., Pešić M; Department of Neurobiology, Institute for Biological Research 'Siniša Stanković'-National Institute of the Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11108 Belgrade, Serbia. |
| Abstrakt: |
Lung cancer remains the leading cause of cancer death globally, with non-small cell lung cancer (NSCLC) accounting for the majority of cases. Multidrug resistance (MDR), often caused by ATP-binding cassette (ABC) transporters, represents a significant obstacle in the treatment of NSCLC. While genetic profiling has an important role in personalized therapy, functional assays that measure cellular responses to drugs are gaining in importance. We developed an automated microplate-based immunofluorescence assay for the evaluation of MDR markers ABCB1, ABCC1, and ABCG2 in cells obtained from NSCLC patients through high-content imaging and image analysis, as part of a functional diagnostic approach. This assay effectively discriminated cancer from non-cancer cells within mixed cultures, which is vital for accurate assessment of changes in MDR marker expression in different cell populations in response to anticancer drugs. Validation was performed using established drug-sensitive (NCI-H460) and drug-resistant (NCI-H460/R) NSCLC cell lines, demonstrating the assay's capacity to distinguish and evaluate different MDR profiles. The obtained results revealed wide-ranging effects of various chemotherapeutic agents on MDR marker expression in different patient-derived NSCLC cultures, emphasizing the need for MDR diagnostics in NSCLC. In addition to being a valuable tool for assessing drug effects on MDR markers in different cell populations, the assay can complement genetic profiling to optimize treatment. Further assay adaptations may extend its application to other cancer types, improving treatment efficacy while minimizing the development of resistance. |
