Disruption of a Plasmodium falciparum patatin-like phospholipase delays male gametocyte exflagellation.

Autor: Pietsch E; Centre for Structural Systems Biology, Hamburg, Germany.; Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.; University of Hamburg, Hamburg, Germany., Niedermüller K; Centre for Structural Systems Biology, Hamburg, Germany.; Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.; University of Hamburg, Hamburg, Germany., Andrews M; Research Centre for Infectious Diseases, School of Biological Sciences, University of Adelaide, Adelaide, South Australia, Australia., Meyer BS; Research Unit for Evolutionary Immunogenomics, Department of Biology, University of Hamburg, Hamburg, Germany., Lenz TL; Research Unit for Evolutionary Immunogenomics, Department of Biology, University of Hamburg, Hamburg, Germany., Wilson DW; Research Centre for Infectious Diseases, School of Biological Sciences, University of Adelaide, Adelaide, South Australia, Australia.; Burnet Institute, Melbourne, Victoria, Australia., Gilberger TW; Centre for Structural Systems Biology, Hamburg, Germany.; Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.; University of Hamburg, Hamburg, Germany., Burda PC; Centre for Structural Systems Biology, Hamburg, Germany.; Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.; University of Hamburg, Hamburg, Germany.
Jazyk: angličtina
Zdroj: Molecular microbiology [Mol Microbiol] 2024 Mar; Vol. 121 (3), pp. 529-542. Date of Electronic Publication: 2023 Dec 22.
DOI: 10.1111/mmi.15211
Abstrakt: An essential process in transmission of the malaria parasite to the Anopheles vector is the conversion of mature gametocytes into gametes within the mosquito gut, where they egress from the red blood cell (RBC). During egress, male gametocytes undergo exflagellation, leading to the formation of eight haploid motile microgametes, while female gametes retain their spherical shape. Gametocyte egress depends on sequential disruption of the parasitophorous vacuole membrane and the host cell membrane. In other life cycle stages of the malaria parasite, phospholipases have been implicated in membrane disruption processes during egress, however their importance for gametocyte egress is relatively unknown. Here, we performed comprehensive functional analyses of six putative phospholipases for their role during development and egress of Plasmodium falciparum gametocytes. We localize two of them, the prodrug activation and resistance esterase (PF3D7_0709700) and the lysophospholipase 1 (PF3D7_1476700), to the parasite plasma membrane. Subsequently, we show that disruption of most of the studied phospholipase genes does neither affect gametocyte development nor egress. The exception is the putative patatin-like phospholipase 3 (PF3D7_0924000), whose gene deletion leads to a delay in male gametocyte exflagellation, indicating an important, albeit not essential, role of this enzyme in male gametogenesis.
(© 2023 The Authors. Molecular Microbiology published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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