An In Vitro Characterization of a PCL-Fibrin Scaffold for Myocardial Repair.

Autor: Jarrell DK; Department of Bioengineering, University of Colorado Denver, Anschutz Medical Campus., Jacot JG; Department of Bioengineering, University of Colorado Denver, Anschutz Medical Campus.; Department of Pediatrics, Children's Hospital Colorado.
Jazyk: angličtina
Zdroj: Materials today. Communications [Mater Today Commun] 2023 Dec; Vol. 37. Date of Electronic Publication: 2023 Nov 21.
DOI: 10.1016/j.mtcomm.2023.107596
Abstrakt: Each year in the United States approximately 10,000 babies are born with a complex congenital heart defect (CHD) requiring surgery in the first year of after birth. Several of these operations require the implantation of a full-thickness heart patch; however, the current patch materials available to pediatric heart surgeons are exclusively non-living and non-degradable, which do not grow with the patient and are prone to fail due to an inability to integrate with the heart. In this work, the goal was to develop a full-thickness, tissue engineered myocardial patch (TEMP) that is made from biodegradable components, strong enough to withstand the mechanical forces of the heart wall, and able to integrate with the heart and drive neotissue formation. Here, a thick and porous electrospun PCL scaffold filled with high-salt PEGylated fibrin was developed. The scaffold was found to be mechanically sufficient for heart wall repair. Vascular cells were able to infiltrate more than halfway through the scaffold in static culture within three weeks. The scaffold maintained pluripotent stem cells for at least four days, supports viable iPSC-derived cardiomyocytes, and fostered tissue thickening in vitro . The TEMP developed here and tested in vitro is promising for the repair of structural CHD and will next be assessed in situ .
Competing Interests: Declaration of interests The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Databáze: MEDLINE
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