The Cousa objective: a long-working distance air objective for multiphoton imaging in vivo.

Autor:	Yu CH; Department of Electrical and Computer Engineering, University of California Santa Barbara, Santa Barbara, CA, USA. chehangyu@ucsb.edu., Yu Y; Department of Electrical and Computer Engineering, University of California Santa Barbara, Santa Barbara, CA, USA., Adsit LM; Department of Molecular, Cellular, and Developmental Biology, University of California Santa Barbara, Santa Barbara, CA, USA., Chang JT; Max Planck Florida Institute for Neuroscience, Jupiter, FL, USA., Barchini J; Max Planck Florida Institute for Neuroscience, Jupiter, FL, USA., Moberly AH; Department of Neuroscience, Yale University, New Haven, CT, USA., Benisty H; Department of Neuroscience, Yale University, New Haven, CT, USA., Kim J; Department of Otolaryngology, Washington University School of Medicine, St. Louis, MO, USA., Young BK; Spencer Center for Vision Research, Byers Eye Institute, School of Medicine, Stanford University, Palo Alto, CA, USA., Heng K; Spencer Center for Vision Research, Byers Eye Institute, School of Medicine, Stanford University, Palo Alto, CA, USA.; Neurosciences Interdepartmental Program, Stanford University, Stanford, CA, USA., Farinella DM; Department of Neuroscience, University of Minnesota, Minneapolis, MN, USA., Leikvoll A; Department of Neuroscience, University of Minnesota, Minneapolis, MN, USA., Pavan R; Department of Biomedical Engineering, University of Minnesota, Minneapolis, MN, USA., Vistein R; Department of Molecular and Comparative Pathobiology, and Zanvyl Krieger Mind/Brain Institute, Johns Hopkins University, Baltimore, MD, USA., Nanfito BR; Solomon H. Snyder Department of Neuroscience, and Zanvyl Krieger Mind/Brain Institute, Johns Hopkins University, Baltimore, MD, USA., Hildebrand DGC; Laboratory of Neural Systems, The Rockefeller University, New York, NY, USA., Otero-Coronel S; Laboratory of Neural Systems, The Rockefeller University, New York, NY, USA.; Laboratory of Neurotechnology and Biophysics, The Rockefeller University, New York, NY, USA.; Kavli Neural Systems Institute, The Rockefeller University, New York, NY, USA., Vaziri A; Laboratory of Neurotechnology and Biophysics, The Rockefeller University, New York, NY, USA.; Kavli Neural Systems Institute, The Rockefeller University, New York, NY, USA., Goldberg JL; Spencer Center for Vision Research, Byers Eye Institute, School of Medicine, Stanford University, Palo Alto, CA, USA., Ricci AJ; Department of Otolaryngology, Stanford University School of Medicine, Stanford University, Stanford, CA, USA.; Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford University, Stanford, CA, USA., Fitzpatrick D; Max Planck Florida Institute for Neuroscience, Jupiter, FL, USA., Cardin JA; Department of Neuroscience, Yale University, New Haven, CT, USA., Higley MJ; Department of Neuroscience, Yale University, New Haven, CT, USA., Smith GB; Department of Neuroscience, University of Minnesota, Minneapolis, MN, USA., Kara P; Department of Neuroscience, University of Minnesota, Minneapolis, MN, USA., Nielsen KJ; Solomon H. Snyder Department of Neuroscience, and Zanvyl Krieger Mind/Brain Institute, Johns Hopkins University, Baltimore, MD, USA., Smith IT; Department of Molecular, Cellular, and Developmental Biology, University of California Santa Barbara, Santa Barbara, CA, USA.; Department of Psychology and Brain Sciences, University of California Santa Barbara, Santa Barbara, CA, USA.; Neuroscience Research Institute, University of California Santa Barbara, Santa Barbara, CA, USA., Smith SL; Department of Electrical and Computer Engineering, University of California Santa Barbara, Santa Barbara, CA, USA. sls@ucsb.edu.; Department of Psychology and Brain Sciences, University of California Santa Barbara, Santa Barbara, CA, USA. sls@ucsb.edu.
Jazyk:	angličtina
Zdroj:	Nature methods [Nat Methods] 2024 Jan; Vol. 21 (1), pp. 132-141. Date of Electronic Publication: 2023 Dec 21.
DOI:	10.1038/s41592-023-02098-1
Abstrakt:	Multiphoton microscopy can resolve fluorescent structures and dynamics deep in scattering tissue and has transformed neural imaging, but applying this technique in vivo can be limited by the mechanical and optical constraints of conventional objectives. Short working distance objectives can collide with compact surgical windows or other instrumentation and preclude imaging. Here we present an ultra-long working distance (20 mm) air objective called the Cousa objective. It is optimized for performance across multiphoton imaging wavelengths, offers a more than 4 mm 2 field of view with submicrometer lateral resolution and is compatible with commonly used multiphoton imaging systems. A novel mechanical design, wider than typical microscope objectives, enabled this combination of specifications. We share the full optical prescription, and report performance including in vivo two-photon and three-photon imaging in an array of species and preparations, including nonhuman primates. The Cousa objective can enable a range of experiments in neuroscience and beyond. (© 2023. The Author(s).)
Databáze:	MEDLINE
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