Insight in lag phase of Listeria monocytogenes during enrichment through proteomic and transcriptomic responses.

Autor: Bannenberg JW; Laboratory of Food Microbiology, Wageningen University and Research, Wageningen, The Netherlands., Boeren S; Laboratory of Biochemistry, Wageningen University and Research, Wageningen, The Netherlands., Zwietering MH; Laboratory of Food Microbiology, Wageningen University and Research, Wageningen, The Netherlands., Abee T; Laboratory of Food Microbiology, Wageningen University and Research, Wageningen, The Netherlands., den Besten HMW; Laboratory of Food Microbiology, Wageningen University and Research, Wageningen, The Netherlands. Electronic address: heidy.denbesten@wur.nl.
Jazyk: angličtina
Zdroj: Food research international (Ottawa, Ont.) [Food Res Int] 2024 Jan; Vol. 175, pp. 113609. Date of Electronic Publication: 2023 Oct 20.
DOI: 10.1016/j.foodres.2023.113609
Abstrakt: The dynamics of the enrichment-based detection procedure of the foodborne pathogen Listeria monocytogenes from food still remains poorly understood. This enrichment is crucial in the reliable detection of this pathogen and more insight into the recovery mechanism during this step is important to advance our understanding of lag phase behaviour during enrichment. In this study we combined transcriptomic and proteomic analyses to better understand the physiological processes within the lag phase of L. monocytogenes during enrichment. Upon transfer of BHI-cultured stationary phase L. monocytogenes cells to half-Fraser enrichment broth (HFB), motility-associated genes and proteins were downregulated, while expression of metal uptake transporters, resuscitation-promoting factors that stimulate growth from dormancy, antibiotic efflux pumps and oxidative stress proteins were upregulated. Next to this, when cells with a heat stress history were cultured in enrichment broth, proteins necessary for recovery were upregulated with functions in DNA-damage repair, protein refolding, cell-wall repair, and zinc transport. Proteomic results pointed to possible factors that support shortening the lag duration, including the addition of 10 µM zinc and the addition of spent HFB containing presumed concentrations of resuscitation-promoting factors. However, these interventions did not lead to biologically relevant reduction of lag phase. Also, when cells were enriched in spent HFB, final cell concentrations were similar to enrichments in fresh HFB, indicating that the enrichment broth seems not to lack critical substrates. Concludingly, this study gives insight into the proteomic changes in the lag phase during enrichment and shows that supplementation of HFB is not the best strategy to optimize the current enrichment method.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 The Author(s). Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
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