m-PIMA™ HIV1/2 VL: A suitable tool for HIV-1 and HIV-2 viral load quantification in West Africa.

Autor: Diop-Ndiaye H; Cheikh Anta Diop University and Bacteriology-Virology UTH Aristide le Dantec, Dakar, Senegal. Electronic address: halimatoudiop@yahoo.fr., Sène PY; Bacteriology-Virology UTH Aristide le Dantec, Dakar, Senegal., Coulibaly K; Division de Lutte contre le SIDA/IST, Dakar, Senegal., Diallo M; Clinton Health Access Initiative, Dakar, Senegal., Diallo S; Bacteriology-Virology UTH Aristide le Dantec, Dakar, Senegal., Diop K; Division de Lutte contre le SIDA/IST, Dakar, Senegal., Sow-Ndoye A; Institut de Recherche en Santé, de surveillance épidémiologique et de Formation, Dakar, Senegal., Fall M; Bacteriology-Virology UTH Aristide le Dantec, Dakar, Senegal., Ndiaye AJS; Institut de Recherche en Santé, de surveillance épidémiologique et de Formation, Dakar, Senegal., Mathebula E; School of Health Systems and Public Health, Faculty of Health Science, University of Pretoria, Pretoria, South Africa., Ba AA; Bacteriology-Virology UTH Aristide le Dantec, Dakar, Senegal., Lejeune C; Clinton Health Access Initiative, Dakar, Senegal., Manga NMP; Bacteriology-Virology UTH Aristide le Dantec, Dakar, Senegal., Camara M; Cheikh Anta Diop University and Bacteriology-Virology UTH Aristide le Dantec, Dakar, Senegal., Ndour CT; Division de Lutte contre le SIDA/IST, Dakar, Senegal., Kane CT; Institut de Recherche en Santé, de surveillance épidémiologique et de Formation, Dakar, Senegal.
Jazyk: angličtina
Zdroj: Journal of virological methods [J Virol Methods] 2024 Feb; Vol. 324, pp. 114872. Date of Electronic Publication: 2023 Dec 19.
DOI: 10.1016/j.jviromet.2023.114872
Abstrakt: Point-of-Care for HIV viral RNA quantification seems to be a complementary strategy to the existing conventional systems. This study evaluated the performance of the m-PIMA™ HIV1/2 Viral Load for the quantification of both HIV-1 and HIV-2 RNA viral load. A total of 555 HIV-1 and 90 HIV-2 samples previously tested by Abbott RealTime HIV-1 (Abbott, Chicago, USA) and Generic HIV-2® Charge virale (Biocentric, France) were tested using the m-PIMA™ HIV1/2 Viral Load at the HIV National Reference lab in Senegal. For HIV-1, Pearson correlation and Bland-Altman plots showed a coefficient r = 0.97 and a bias of -0.11 log 10 copies/ml (95% confidence interval [CI]: -0.086 to -0.133 log 10 copies/ml) for the m-PIMA™ HIV1/2 Viral Load, respectively. Sensitivity and specificity at 3 log 10 copies/ml (threshold of virological failure) were 93.6% (95%[CI]: 91.5% to 95.6%) and 99.1% (95%[CI]: 98.3% to 99.9%), respectively. For HIV-2, a correlation of r = 0.95 was also noted with a bias of - 0.229 log 10 copies/ml (95%[CI]: -0.161 to -0.297 log 10 copies/ml). Sensitivity and specificity at 3 log 10 copies/ml were 97.6% (95%[CI]: 94.3% to 100%) and 93.9% (95%[CI]: 88.9% to 98.8%), respectively. These results confirmed that m-PIMA™ HIV1/2 VL could be a good alternative for HIV-1 and HIV-2 viral load testing in decentralized settings in Senegal.
Competing Interests: Declaration of Competing Interest The authors do not declare any conflict of interest.
(Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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