Novel lactoferrin-conjugated gallium complex to treat Pseudomonas aeruginosa wound infection.

Autor: Valappil SP; Chester Medical School, University of Chester, Bache Hall, Countess View, Chester CH2 1BR, United Kingdom; Institute of Population Health, University of Liverpool, Research Wing, Daulby Street, Liverpool L69 3GN, United Kingdom. Electronic address: s.valappil@chester.ac.uk., Abou Neel EA; Preventive and Restorative Dentistry Department, College of Dental Medicine, University of Sharjah, Sharjah, United Arab Emirates; UCL Eastman Dental Institute, Biomaterials & Tissue Engineering Division, Royal Free Hospital, Rowland Hill Street, London, UK., Zakir Hossain KM; Department of Chemistry, University of Bath, Claverton Down, Bath BA2 7AY, UK., Paul W; Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram 695012, India., Cherukaraveedu D; Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram 695012, India., Wade B; Institute of Population Health, University of Liverpool, Research Wing, Daulby Street, Liverpool L69 3GN, United Kingdom., Ansari TI; Northwick Park Institute for Medical Research, Watford Road, Harrow HA1 3UJ, United Kingdom., Hope CK; Institute of Population Health, University of Liverpool, Research Wing, Daulby Street, Liverpool L69 3GN, United Kingdom., Higham SM; Institute of Population Health, University of Liverpool, Research Wing, Daulby Street, Liverpool L69 3GN, United Kingdom., Sharma CP; Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram 695012, India.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2024 Feb; Vol. 258 (Pt 1), pp. 128838. Date of Electronic Publication: 2023 Dec 19.
DOI: 10.1016/j.ijbiomac.2023.128838
Abstrakt: Pseudomonas aeruginosa is one of the leading causes of opportunistic infections such as chronic wound infection that could lead to multiple organ failure and death. Gallium (Ga 3+ ) ions are known to inhibit P. aeruginosa growth and biofilm formation but require carrier for localized controlled delivery. Lactoferrin (LTf), a two-lobed protein, can deliver Ga 3+ at sites of infection. This study aimed to develop a Ga-LTf complex for the treatment of wound infection. The characterisation of the Ga-LTf complex was conducted using differential scanning calorimetry (DSC), Infra-Red (FTIR) and Inductive Coupled Plasma Optical Emission Spectrometry (ICP-OES). The antibacterial activity was assessed by agar disc diffusion, liquid broth and biofilm inhibition assays using the colony forming units (CFUs). The healing capacity and biocompatibility were evaluated using a P.aeruginosa infected wound in a rat model. DSC analyses showed thermal transition consistent with apo-lactoferrin; FTIR confirmed the complexation of gallium to lactoferrin. ICP-OES confirmed the controlled local delivery of Ga 3+ . Ga-LTf showed a 0.57 log 10 CFUs reduction at 24 h compared with untreated control in planktonic liquid broth assay. Ga-LTf showed the highest antibiofilm activity with a 2.24 log 10 CFUs reduction at 24 h. Furthermore, Ga-LTf complex is biocompatible without any adverse effect on brain, kidney, liver and spleen of rats tested in this study. Ga-LTf can be potentially promising novel therapeutic agent to treat pathogenic bacterial infections.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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