A novel goose-origin Tembusu virus exhibits pathogenicity in day-old chicks with evidence of direct contact transmission.

Autor:	Liu M; Department of Veterinary Medicine, National Taiwan University, Taipei, Taiwan., Chen YY; Department of Veterinary Medicine, National Taiwan University, Taipei, Taiwan., Twu NC; Department of Veterinary Medicine, National Chiayi University, Chiayi, Taiwan., Wu MC; Department of Veterinary Medicine, National Taiwan University, Taipei, Taiwan., Fang ZS; Department of Veterinary Medicine, National Taiwan University, Taipei, Taiwan., Dubruel A; Department of Veterinary Medicine, National Taiwan University, Taipei, Taiwan., Chang SC; Department of Biochemical Science and Technology, National Taiwan University, Taipei, Taiwan., Wu CF; Department of Veterinary Medicine, National Chiayi University, Chiayi, Taiwan., Lo DY; Department of Veterinary Medicine, National Chiayi University, Chiayi, Taiwan., Chen HW; Department of Veterinary Medicine, National Taiwan University, Taipei, Taiwan; Animal Resource Center, National Taiwan University, Taipei, Taiwan. Electronic address: winnichen@ntu.edu.tw.
Jazyk:	angličtina
Zdroj:	Poultry science [Poult Sci] 2024 Feb; Vol. 103 (2), pp. 103332. Date of Electronic Publication: 2023 Nov 30.
DOI:	10.1016/j.psj.2023.103332
Abstrakt:	In late 2020, an outbreak of Tembusu virus (TMUV)-associated disease occurred in a 45-day-old white Roman geese flock in Taiwan. Here, we present the identification and isolation of a novel goose-origin TMUV strain designated as NTU/C225/2020. The virus was successfully isolated using minimal-pathogen-free duck embryos. Phylogenetic analysis of the polyprotein gene showed that NTU/C225/2020 clustered together with the earliest isolates from Malaysia and was most closely related to the first Taiwanese TMUV strain, TP1906. Genomic analysis revealed significant amino acid variations among TMUV isolates in NS1 and NS2A protein regions. In the present study, we characterized the NTU/C225/2020 culture in duck embryos, chicken embryos, primary duck embryonated fibroblasts, and DF-1 cells. All host systems were susceptible to NTU/C225/2020 infection, with observable lesions. In addition, animal experiments showed that the intramuscular inoculation of NTU/C225/2020 resulted in growth retardation and hyperthermia in day-old chicks. Gross lesions in the infected chicks included hepatomegaly, hyperemic thymus, and splenomegaly. Viral loads and histopathological damage were displayed in various tissues of both inoculated and naïve co-housed chicks, confirming the direct chick-to-chick contact transmission of TMUV. This is the first in vivo study of a local TMUV strain in Taiwan. Our findings provide essential information for TMUV propagation and suggest a potential risk of disease outbreak in chicken populations. (Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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