Genetic Analysis of the Emerging Citrus Yellow Vein Clearing Virus Reveals a Divergent Virus Population in American Isolates.

Autor:	Abrahamian P; Plant Pathogen Confirmatory Diagnostics Laboratory, PPQ S&T, USDA-APHIS, Laurel, MD 20708., Tian T; Plant Pest Diagnostics Center, California Department of Food and Agriculture, Sacramento, CA 95832., Posis K; Plant Pest Diagnostics Center, California Department of Food and Agriculture, Sacramento, CA 95832., Guo Y; Plant Pest Diagnostics Center, California Department of Food and Agriculture, Sacramento, CA 95832., Yu D; Plant Pest Diagnostics Center, California Department of Food and Agriculture, Sacramento, CA 95832., Blomquist CL; Plant Pest Diagnostics Center, California Department of Food and Agriculture, Sacramento, CA 95832., Wei G; Plant Pathogen Confirmatory Diagnostics Laboratory, PPQ S&T, USDA-APHIS, Laurel, MD 20708., Adducci B; Plant Pathogen Confirmatory Diagnostics Laboratory, PPQ S&T, USDA-APHIS, Laurel, MD 20708., Vidalakis G; Department of Microbiology and Plant Pathology, University of California, Riverside, CA 92521., Bodaghi S; Department of Microbiology and Plant Pathology, University of California, Riverside, CA 92521., Osman F; Department of Plant Pathology, University of California, Davis, CA 95616., Roy A; Molecular Plant Pathology Laboratory, Beltsville Agriculture Research Center, USDA-ARS, Beltsville, MD 20705., Nunziata SO; Plant Pathogen Confirmatory Diagnostics Laboratory, PPQ S&T, USDA-APHIS, Laurel, MD 20708., Nakhla M; Plant Pathogen Confirmatory Diagnostics Laboratory, PPQ S&T, USDA-APHIS, Laurel, MD 20708., Mavrodieva V; Plant Pathogen Confirmatory Diagnostics Laboratory, PPQ S&T, USDA-APHIS, Laurel, MD 20708., Rivera Y; Plant Pathogen Confirmatory Diagnostics Laboratory, PPQ S&T, USDA-APHIS, Laurel, MD 20708.
Jazyk:	angličtina
Zdroj:	Plant disease [Plant Dis] 2024 Jun; Vol. 108 (6), pp. 1544-1554. Date of Electronic Publication: 2024 May 18.
DOI:	10.1094/PDIS-09-23-1963-RE
Abstrakt:	Citrus yellow vein clearing virus is a previously reported citrus virus from Asia with widespread distribution in China. In 2022, the California Department of Food and Agriculture conducted a multipest citrus survey targeting multiple citrus pathogens including citrus yellow vein clearing virus (CYVCV). In March 2022, a lemon tree with symptoms of vein clearing, chlorosis, and mottling in a private garden in the city of Tulare, California, tested positive for CYVCV, which triggered an intensive survey in the surrounding areas. A total of 3,019 plant samples, including citrus and noncitrus species, were collected and tested for CYVCV using conventional reverse transcription polymerase chain reaction, reverse transcription quantitative polymerase chain reaction, and Sanger sequencing. Five hundred eighty-six citrus trees tested positive for CYVCV, including eight citrus species not previously recorded infected under field conditions. Comparative genomic studies were conducted using 17 complete viral genomes. Sequence analysis revealed two major phylogenetic groups. Known Asian isolates and five California isolates from this study made up the first group, whereas all other CYVCV isolates from California formed a second group, distinct from all worldwide isolates. Overall, the CYVCV population shows rapid expansion and high differentiation indicating a population bottleneck typical of a recent introduction into a new geographic area. Competing Interests: The author(s) declare no conflict of interest.
Databáze:	MEDLINE
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