Paraoxonase and arylesterase activity of paraoxonase 1 and oxidative stress parameters in cervical intraepithelial neoplasia.

Autor:	Butorac D; Clinic of Gynecology and Obstetrics, Sestre milosrdnice University Hospital Center, Zagreb, Croatia., Ćelap I; Department of Clinical Chemistry, Sestre milosrdnice University Hospital Center, Zagreb, Croatia.; Department of Medical biochemistry and Hematology, Faculty of Pharmacy and Biochemistry, University of Zagreb, Zagreb, Croatia., Kačkov Maslać S; Medical biochemistry laboratory, Polyclinic Bonifarm, Zagreb, Croatia., Miletić T; Polyclinic Aviva, Zagreb, Croatia., Tomašković AH; Department of Medical biochemistry and Hematology, Faculty of Pharmacy and Biochemistry, University of Zagreb, Zagreb, Croatia., Turčić P; Department of Pharmacology, Faculty of Pharmacy and Biochemistry, University of Zagreb, Zagreb, Croatia., Rašić D; Unit of Toxicology, Institute for Medical Research and Occupational Health, Zagreb, Croatia., Stojanović I; Clinic of Gynecology and Obstetrics, Sestre milosrdnice University Hospital Center, Zagreb, Croatia., Rajković MG; Department of Medical biochemistry and Hematology, Faculty of Pharmacy and Biochemistry, University of Zagreb, Zagreb, Croatia.
Jazyk:	angličtina
Zdroj:	Biochemia medica [Biochem Med (Zagreb)] 2024 Feb 15; Vol. 34 (1), pp. 030701. Date of Electronic Publication: 2023 Dec 15.
DOI:	10.11613/BM.2024.010701
Abstrakt:	Introduction: Paraoxonase 1 (PON1) is the enzyme that removes carcinogenic radicals from lipids. The aim of the study was to investigate the differences in PON1 activity and oxidation stress parameters between patients with cervical intraepithelial neoplasia (CIN) and healthy controls. Materials and Methods: The study included 65 women with CIN and 109 healthy women. Lipid parameters were determined on Cobas Integra 400 plus (Roche, Mannheim, Germany). Tiols and reduced glutathione (GSH) were determined spectrophotometric using Eliman reagent. Activity of PON1 was assessed with two substrates, paraoxon and phenylacetate by spectrophotometric method. Malondialdehyde (MDA) was determined by high performance liquid chromatography (Shimadzu Corporation, Kyoto, Japan). Mann-Whitney-test, t-test, χ2-test, correlation and logistic regression was used in statistical analysis. P < 0.05 was considered statistically significant. Results: The basal (P = 0.929) and NaCl-stimulated (P = 0.985) PON1 activity and activities standardised on the concentration of high-density lipoprotein (HDL; P = 0.076; P = 0.065, respectively) and apolipoprotein AI (apo AI; P = 0.444; P = 0.499, respectively) as well as PON1 phenotypes (P = 0.842) did not differ significantly between the groups. The PON1 arylesterase activity (53±19 kU/L vs. 77±17 kU/L; P < 0.001) and HDL-standardized activity (37 (28-44) kU/mmol vs . 43 (37-50) kU/mmol; P < 0.001) and apoAI (29±11 kU/g vs . 44±11 kU/g; P < 0.001) was significantly reduced in the CIN group. The concentration of the thiol groups was similar (P = 0.519), of MDA was lower (0.39 (0.27-0.55) µmol/L vs . 0.76 (0.57-1.15) µmol/L; P < 0.001) and of GSH was higher (112.0 (66.0-129.6) µg/mL vs . 53.4 (34.8-134.4) µg/mL; P < 0.001) in the CIN group. Conclusion: Reduced PON1 arylesterase activity, lower MDA and higher GSH concentration were observed in CIN patients. Competing Interests: Potential conflict of interest None declared. (Croatian Society of Medical Biochemistry and Laboratory Medicine.)
Databáze:	MEDLINE
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