Interaction mechanism of a cysteine protease inhibitor, odanacatib, with human serum albumin: In vitro and bioinformatics studies.
Autor: | Asngari NJM; Department of Oral & Craniofacial Sciences, Faculty of Dentistry, Universiti Malaya, 50603 Kuala Lumpur, Malaysia., Bakar KA; Department of Biological Sciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia., Feroz SR; Department of Biological Sciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia., Razak FA; Department of Oral & Craniofacial Sciences, Faculty of Dentistry, Universiti Malaya, 50603 Kuala Lumpur, Malaysia; Adjunct Professor, Faculty of Dentistry, Universitas Padjadjaran, Bandung, Indonesia., Halim AAA; Department of Oral & Craniofacial Sciences, Faculty of Dentistry, Universiti Malaya, 50603 Kuala Lumpur, Malaysia. Electronic address: adyaniazizah@um.edu.my. |
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Jazyk: | angličtina |
Zdroj: | Biophysical chemistry [Biophys Chem] 2024 Feb; Vol. 305, pp. 107140. Date of Electronic Publication: 2023 Nov 10. |
DOI: | 10.1016/j.bpc.2023.107140 |
Abstrakt: | Odanacatib (ODN) is a selective cathepsin K inhibitor that acts as an anti-resorptive agent to treat osteoporosis. ODN is also found effective in reducing the effect of severe periodontitis. The interaction between ODN and human serum albumin (HSA) was investigated using spectroscopic, microscopic, and in silico approaches to characterize their binding. The fluorescence intensity of HSA increased upon the addition of increasing concentrations of ODN accompanied by blueshift in the fluorescence spectrum, which suggested hydrophobic formation around the microenvironment of the fluorophores upon ODN binding. A moderate binding affinity was obtained for ODN-HSA binding, with binding constant (K Competing Interests: Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Adyani Azizah Abd Halim reports financial support was provided by University of Malaya. (Copyright © 2023 Elsevier B.V. All rights reserved.) |
Databáze: | MEDLINE |
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