Design of a Biohybrid Materials Circuit with Binary Decoder Functionality.

Autor: Mohsenin H; Signalling Research Centres BIOSS and CIBSS, University of Freiburg, Schänzlestraße 18, 79104, Freiburg, Germany.; INM - Leibniz Institute for New Materials, Campus D2 2, 66123, Saarbrücken, Germany.; Faculty of Biology, University of Freiburg, Schänzlestraße 1, 79104, Freiburg, Germany., Wagner HJ; Signalling Research Centres BIOSS and CIBSS, University of Freiburg, Schänzlestraße 18, 79104, Freiburg, Germany.; Faculty of Biology, University of Freiburg, Schänzlestraße 1, 79104, Freiburg, Germany.; Spemann Graduate School of Biology and Medicine (SGBM), University of Freiburg, Albertstraße 19a, 79104, Freiburg, Germany., Rosenblatt M; Institute of Physics and Freiburg Center for Data Analysis and Modelling (FDM), University of Freiburg, Hermann-Herder-Straße 3, 79104, Freiburg, Germany., Kemmer S; Faculty of Biology, University of Freiburg, Schänzlestraße 1, 79104, Freiburg, Germany.; Institute of Physics and Freiburg Center for Data Analysis and Modelling (FDM), University of Freiburg, Hermann-Herder-Straße 3, 79104, Freiburg, Germany., Drepper F; Faculty of Biology, University of Freiburg, Schänzlestraße 1, 79104, Freiburg, Germany., Huesgen P; Faculty of Biology, University of Freiburg, Schänzlestraße 1, 79104, Freiburg, Germany.; CIBSS - Centre for Integrative Biological Signalling Studies, University of Freiburg, Schänzlestraße 18, 79104, Freiburg, Germany., Timmer J; Signalling Research Centres BIOSS and CIBSS, University of Freiburg, Schänzlestraße 18, 79104, Freiburg, Germany.; Institute of Physics and Freiburg Center for Data Analysis and Modelling (FDM), University of Freiburg, Hermann-Herder-Straße 3, 79104, Freiburg, Germany., Weber W; Signalling Research Centres BIOSS and CIBSS, University of Freiburg, Schänzlestraße 18, 79104, Freiburg, Germany.; INM - Leibniz Institute for New Materials, Campus D2 2, 66123, Saarbrücken, Germany.; Faculty of Biology, University of Freiburg, Schänzlestraße 1, 79104, Freiburg, Germany.; Spemann Graduate School of Biology and Medicine (SGBM), University of Freiburg, Albertstraße 19a, 79104, Freiburg, Germany.; Saarland University, Department of Materials Science and Engineering, Campus D2 2, 66123, Saarbrücken, Germany.
Jazyk: angličtina
Zdroj: Advanced materials (Deerfield Beach, Fla.) [Adv Mater] 2024 Apr; Vol. 36 (14), pp. e2308092. Date of Electronic Publication: 2024 Jan 05.
DOI: 10.1002/adma.202308092
Abstrakt: Synthetic biology applies concepts from electrical engineering and information processing to endow cells with computational functionality. Transferring the underlying molecular components into materials and wiring them according to topologies inspired by electronic circuit boards has yielded materials systems that perform selected computational operations. However, the limited functionality of available building blocks is restricting the implementation of advanced information-processing circuits into materials. Here, a set of protease-based biohybrid modules the bioactivity of which can either be induced or inhibited is engineered. Guided by a quantitative mathematical model and following a design-build-test-learn (DBTL) cycle, the modules are wired according to circuit topologies inspired by electronic signal decoders, a fundamental motif in information processing. A 2-input/4-output binary decoder for the detection of two small molecules in a material framework that can perform regulated outputs in form of distinct protease activities is designed. The here demonstrated smart material system is strongly modular and can be used for biomolecular information processing for example in advanced biosensing or drug delivery applications.
(© 2024 The Authors. Advanced Materials published by Wiley‐VCH GmbH.)
Databáze: MEDLINE
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