A robust method for simultaneous determination of eight B vitamins in human serum by liquid chromatography tandem mass spectrometry.

Autor: Huo YM; Hangzhou Adicon Clinical Laboratories Co., Ltd, Hangzhou, P. R. China., Zhang SQ; Department of Chemistry, College of Sciences, Northeastern University, Shenyang, P. R. China., Wu GP; Hangzhou Adicon Clinical Laboratories Co., Ltd, Hangzhou, P. R. China., Shan HB; Hangzhou Adicon Clinical Laboratories Co., Ltd, Hangzhou, P. R. China., Pan C; Hangzhou Adicon Clinical Laboratories Co., Ltd, Hangzhou, P. R. China.
Jazyk: angličtina
Zdroj: Journal of separation science [J Sep Sci] 2024 Jan; Vol. 47 (1), pp. e2300576. Date of Electronic Publication: 2023 Dec 20.
DOI: 10.1002/jssc.202300576
Abstrakt: The level of vitamin B group in human serum is an important index of human health. Among B vitamins, cyanocobalamin in serum is unstable and its content is extremely low. Rapid and simultaneous detection of multiple B vitamins including cyanocobalamin is a challenge. Herein, we have developed a rapid and stable method that can realize the determination of thiamine, riboflavin, nicotinamide, pantothenic acid, pyridoxic acid, biotin, 5-methyltetrahydrofolate, and cyanocobalamin simultaneously in 6 min. The method was established based on protein precipitation with methanol and then chromatographic separation was achieved using Waters acquity ultra-high-performance liquid chromatography high strength silica T3 column, which was stable and sensitive especially for cyanocobalamin. Limit of quantification, precision, trueness, and matrix effect were validated according to the European Medicines Agency and United States Food and Drug guidelines and Clinical and Laboratory Standards Institute guidelines on bioanalytical method. The limit of quantification for thiamine, riboflavin, nicotinamide, pantothenic acid, pyridoxic acid, biotin, 5-methyltetrahydrofolate, and cyanocobalamin was 0.4, 0.4, 0.8, 2.0, 0.4, 0.1, 0.4, and 0.04 ng/mL separately, respectively. Intra- and interday precisions were 1.1%-12.4% and 2.0%-13.5%, respectively. The relative errors were between 0.3% and 13.3%, and the matrix effects were between 2.6% and 10.4%.
(© 2023 Wiley-VCH GmbH.)
Databáze: MEDLINE
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