Environmental detection of eumycetoma pathogens using multiplex real-time PCR for soil DNA in Sennar State, Sudan.

Autor: Hashizume H; School of Tropical Medicine and Global Health, Nagasaki University, 1-12-4 Sakamoto, Nagasaki, 852-8523, Japan.; Department of Ecoepidemiology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, 1-12-4 Sakamoto, Nagasaki, 852-8523, Japan., Taga S; Department of Ecoepidemiology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, 1-12-4 Sakamoto, Nagasaki, 852-8523, Japan., Sakata MK; Graduate School of Human Development and Environment, Kobe University, 3-11 Tsurukabuto, Nada-Ku, Kobe, 657-8501, Japan.; Research Faculty of Agriculture, Hokkaido University, Kita-9, Nishi-9, Kita-Ku, Sapporo, Hokkaido, 060-8589, Japan., Hussein M; Mycetoma Research Center, University of Khartoum, P.O. Box 102, Khartoum, Sudan.; Tumors Therapy and Cancer Research Center, Molecular Biology Unit, Shendi University, P.O .Box 142-143, Shendi, Sudan., Siddig EE; Mycetoma Research Center, University of Khartoum, P.O. Box 102, Khartoum, Sudan.; Faculty of Medical Laboratory Sciences, Unit of Basic Medical Sciences, University of Khartoum, Khartoum, Sudan., Minamoto T; Graduate School of Human Development and Environment, Kobe University, 3-11 Tsurukabuto, Nada-Ku, Kobe, 657-8501, Japan., Fahal AH; Mycetoma Research Center, University of Khartoum, P.O. Box 102, Khartoum, Sudan., Kaneko S; School of Tropical Medicine and Global Health, Nagasaki University, 1-12-4 Sakamoto, Nagasaki, 852-8523, Japan. skaneko@nagasaki-u.ac.jp.; Department of Ecoepidemiology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, 1-12-4 Sakamoto, Nagasaki, 852-8523, Japan. skaneko@nagasaki-u.ac.jp.
Jazyk: angličtina
Zdroj: Tropical medicine and health [Trop Med Health] 2023 Dec 19; Vol. 51 (1), pp. 71. Date of Electronic Publication: 2023 Dec 19.
DOI: 10.1186/s41182-023-00563-3
Abstrakt: Background: Mycetoma is a chronic disease affecting the skin and subcutaneous tissue endemic in the tropical and subtropical regions. Several bacteria and fungi can cause mycetoma, but fungal mycetoma (eumycetoma) is challenging because the treatment requires a combination of a long-term antifungal agent and surgery. Although the transmission route has not yet been elucidated, infection from the soil is a leading hypothesis. However, there are few soil investigation studies, and the geographical distribution of mycetoma pathogens is not well documented. Here, we used multiplex real-time PCR technology to identify three fungal species from soil samples.
Methods: In total, 64 DNA samples were extracted from soil collected in seven villages in an endemic area in Sennar State, Sudan, in 2019. Primers and fluorescent probes specifically targeting the ribosomal DNA of Madurella mycetomatis, Falciformispora senegalensis, and F. tompkinsii were designed.
Results: Multiplex real-time PCR was performed and identified the major pathogen, M. mycetomatis that existed in most sites (95%). In addition, two other pathogens were identified from some sites. This is the first report on the use of this technique for identifying the eumycetoma causative microorganisms.
Conclusions: This study demonstrated that soil DNA investigation can elucidate the risk area of mycetoma-causative agents. The results will contribute to the design of prevention measures, and further large-scale studies may be effective in understanding the natural habitats of mycetoma pathogens.
(© 2023. The Author(s).)
Databáze: MEDLINE
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