Functional groups matter: metabolomics analysis of Escherichia coli exposed to trans-cinnamic acid and its derivatives unveils common and unique targets.

Autor: Onat-Taşdelen KA; Graduate School of Natural and Applied Sciences, Biology Program, Muğla Sıtkı Koçman University, Muğla, Türkiye., Öztürkel-Kabakaş H; Graduate School of Natural and Applied Sciences, Biology Program, Muğla Sıtkı Koçman University, Muğla, Türkiye., Yüksektepe E; Vocational School of Health Services, Pathology Laboratory Techniques Program, Fenerbahçe University, İstanbul, Türkiye., Çatav ŞS; College of Sciences, Department of Biology, Muğla Sıtkı Koçman University, Muğla, Türkiye., Güzel G; Graduate School of Natural and Applied Sciences, Chemistry Program, Muğla Sıtkı Koçman University, Muğla, Türkiye., Çöl B; College of Sciences, Department of Biology, Muğla Sıtkı Koçman University, Muğla, Türkiye.; Biotechnology Research Center, Muğla Sıtkı Koçman University, Muğla, Türkiye., Kim H; College of Natural Sciences, Department of Chemistry, Sejong University, Seoul, South Korea., Chae YK; College of Natural Sciences, Department of Chemistry, Sejong University, Seoul, South Korea., Elgin ES; College of Sciences, Department of Chemistry, Muğla Sıtkı Koçman University, Muğla, Türkiye. selgin@mu.edu.tr.; Research Laboratories Center, Metabolism Laboratory, Muğla Sıtkı Koçman University, Muğla, Türkiye. selgin@mu.edu.tr.
Jazyk: angličtina
Zdroj: World journal of microbiology & biotechnology [World J Microbiol Biotechnol] 2023 Dec 20; Vol. 40 (2), pp. 47. Date of Electronic Publication: 2023 Dec 20.
DOI: 10.1007/s11274-023-03841-8
Abstrakt: Phenolic acids are derivatives of benzoic and cinnamic acids, which possess important biological activities at certain concentrations. Trans-cinnamic acid (t-CA) and its derivatives, such as p-coumaric acid (p-CA) and ferulic acid (FA) have been shown to have antibacterial activity against various Gram-positive and -negative bacteria. However, there is limited information available concerning the antibacterial mode of action of these phenolic acids. In this study, we aimed to ascertain metabolic alterations associated with exposure to t-CA, p-CA, and FA in Escherichia coli BW25113 using a nuclear magnetic resonance (NMR)-based metabolomics approach. The results showed that t-CA, p-CA, and FA treatments led to significant changes (p < 0.05) in the concentration of 42, 55, and 74% of the identified metabolites in E. coli, respectively. Partial least-squares discriminant analysis (PLS-DA) revealed a clear separation between control and phenolic acid groups with regard to metabolic response. Moreover, it was found that FA and p-CA treatment groups were clustered closely together but separated from the t-CA treatment group. Arginine, putrescine, cadaverine, galactose, and sucrose had the greatest impact on group differentiation. Quantitative pathway analysis demonstrated that arginine and proline, pyrimidine, glutathione, and galactose metabolisms, as well as aminoacyl-tRNA and arginine biosyntheses, were markedly affected by all phenolic acids. Finally, the H 2 O 2 content of E. coli cells was significantly increased in response to t-CA and p-CA whereas all phenolic acids caused a dramatic increase in the number of apurinic/apyrimidinic sites. Overall, this study suggests that the metabolic response of E. coli cells to t-CA is relatively different from that to p-CA and FA. However, all phenolic acids had a certain impact on oxidative/antioxidant status, genomic stability, arginine-related pathways, and nucleic acid metabolism.
(© 2023. The Author(s), under exclusive licence to Springer Nature B.V.)
Databáze: MEDLINE
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