Discovery of Three Bemisia tabaci Effectors and Their Effect on Gene Expression in Planta.

Autor:	van Kleeff PJM; Green Life Sciences Research Cluster, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam 1098 XH, The Netherlands., Mastop M; Green Life Sciences Research Cluster, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam 1098 XH, The Netherlands., Sun P; Green Life Sciences Research Cluster, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam 1098 XH, The Netherlands., Dangol S; Green Life Sciences Research Cluster, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam 1098 XH, The Netherlands., van Doore E; Green Life Sciences Research Cluster, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam 1098 XH, The Netherlands., Dekker HL; Laboratory for Mass Spectrometry of Biomolecules, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam 1098 XH, The Netherlands., Kramer G; Laboratory for Mass Spectrometry of Biomolecules, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam 1098 XH, The Netherlands., Lee S; Molecular Phytobacteriology Laboratory, Infectious Disease Research Center, KRIBB, Daejeon 34141, South Korea., Ryu CM; Molecular Phytobacteriology Laboratory, Infectious Disease Research Center, KRIBB, Daejeon 34141, South Korea., de Vos M; Keygene N.V., Wageningen 6708 PW, The Netherlands., Schuurink RC; Green Life Sciences Research Cluster, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam 1098 XH, The Netherlands.
Jazyk:	angličtina
Zdroj:	Molecular plant-microbe interactions : MPMI [Mol Plant Microbe Interact] 2024 Apr; Vol. 37 (4), pp. 380-395. Date of Electronic Publication: 2024 Apr 23.
DOI:	10.1094/MPMI-04-23-0044-R
Abstrakt:	Bemisia tabaci (whitefly) is a polyphagous agroeconomic pest species complex. Two members of this species complex, Mediterranean (MED) and Middle-East-Asia Minor 1 (MEAM1), have a worldwide distribution and have been shown to manipulate plant defenses through effectors. In this study, we used three different strategies to identify three MEAM1 proteins that can act as effectors. Effector B1 was identified using a bioinformatics-driven effector-mining strategy, whereas effectors S1 and P1 were identified in the saliva of whiteflies collected from artificial diet and in phloem exudate of tomato on which nymphs were feeding, respectively. These three effectors were B. tabaci specific and able to increase whitefly fecundity when transiently expressed in tobacco plants ( Nicotiana tabacum ). Moreover, they reduced growth of Pseudomonas syringae pv. tabaci in Nicotiana benthamiana . All three effectors changed gene expression in planta, and B1 and S1 also changed phytohormone levels. Gene ontology and KEGG pathway enrichment analysis pinpointed plant-pathogen interaction and photosynthesis as the main enriched pathways for all three effectors. Our data thus show the discovery and validation of three new B. tabaci MEAM1 effectors that increase whitefly fecundity and modulate plant immunity. [Formula: see text] Copyright © 2024 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license. Competing Interests: The author(s) declare no conflict of interest.
Databáze:	MEDLINE
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