Multicenter molecular investigation of recurrent Escherichia coli bacteriuria in dogs.

Autor: LeCuyer TE; Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA, USA; Washington Animal Disease Diagnostic Laboratory, Washington State University, Pullman, WA, USA. Electronic address: tlecuyer@ucdavis.edu., Sellon RK; Veterinary Clinical Sciences, College of Veterinary Medicine, Washington State University, Pullman, WA, USA., Byrne BA; Pathology, Microbiology, and Immunology, School of Veterinary Medicine, University of California, Davis, CA, USA., Daniels JB; Veterinary Clinical Sciences, College of Veterinary Medicine, College of Veterinary Medicine, The Ohio State University, Columbus, OH, USA., Diaz-Campos DV; Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA, USA; Washington Animal Disease Diagnostic Laboratory, Washington State University, Pullman, WA, USA., Hendrix GK; Comparative Pathobiology, College of Veterinary Medicine, Purdue University, West Lafayette, IN, USA; Indiana Animal Disease Diagnostic Laboratory, West Lafayette, IN, USA., Burbick CR; Veterinary Diagnostic Laboratory, North Dakota State University, Fargo, ND, USA., Besser TE; Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA, USA., Davis MA; Veterinary Clinical Sciences, College of Veterinary Medicine, Washington State University, Pullman, WA, USA; Paul G. Allen School for Global Animal Health, College of Veterinary Medicine, Washington State University, Pullman, WA, USA.
Jazyk: angličtina
Zdroj: Veterinary microbiology [Vet Microbiol] 2024 Jan; Vol. 288, pp. 109914. Date of Electronic Publication: 2023 Dec 07.
DOI: 10.1016/j.vetmic.2023.109914
Abstrakt: Escherichia coli is the most common cause of recurrent urinary tract infection (UTI) in dogs. UTI recurrence comprises of persistent, unresolved E. coli infection or reinfection with a different strain of E. coli. Differentiating between these processes is clinically important but is often impossible with routine diagnostics. We tested the hypothesis that most recurrent canine E. coli bacteriuria is due to recurrence of the same E. coli strain involved in the initial infection. Molecular typing was performed on 98 urinary E. coli isolated from dogs with recurrent bacteriuria from five veterinary diagnostic laboratories in the United States. Of the 42 dogs in this study with multiple E. coli bacteriuria observations, a single strain of E. coli caused recurrent bacteriuria in 26 (62 %) dogs, in some cases on multiple occasions for prolonged periods of time (up to eight months). A single E. coli strain was detected during both subclinical bacteriuria and clinically-apparent UTI in three dogs. Isolates with the P-fimbrial adhesin genes papA and papC were associated with recurrence by the same strain of E. coli. Multiple isolations of a single strain of E. coli associated with recurrent bacteriuria suggests that E. coli may be maintained within the urinary tract of some dogs for prolonged periods of time. In some patients, the same strain can cause both clinical UTI and subclinical bacteriuria. This indicates that in dogs, the urinary bladder may serve as a subclinical, long-term reservoir of E. coli that may cause clinical UTI in the future.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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