Gene expression changes throughout the life cycle allow a bacterial plant pathogen to persist in diverse environmental habitats.

Autor: de Pedro-Jové R; Department of Genetics, Microbiology and Statistics, University of Barcelona, Barcelona, Catalonia, Spain.; Centre for Research in Agricultural Genomics (CSIC-IRTA-UAB-UB), Bellaterra, Catalonia, Spain., Corral J; Centre for Research in Agricultural Genomics (CSIC-IRTA-UAB-UB), Bellaterra, Catalonia, Spain., Rocafort M; Centre for Research in Agricultural Genomics (CSIC-IRTA-UAB-UB), Bellaterra, Catalonia, Spain., Puigvert M; Department of Genetics, Microbiology and Statistics, University of Barcelona, Barcelona, Catalonia, Spain.; Centre for Research in Agricultural Genomics (CSIC-IRTA-UAB-UB), Bellaterra, Catalonia, Spain., Azam FL; Department of Genetics, Microbiology and Statistics, University of Barcelona, Barcelona, Catalonia, Spain.; Centre for Research in Agricultural Genomics (CSIC-IRTA-UAB-UB), Bellaterra, Catalonia, Spain., Vandecaveye A; Área Biología Molecular, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario and Instituto de Biología Molecular y Celular de Rosario, Consejo Nacional de Investigaciones Científicas y Técnicas (IBR-UNR-CONICET), Rosario, Santa Fe, Argentina., Macho AP; Shanghai Centre for Plant Stress Biology, CAS Centre for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai, China., Balsalobre C; Department of Genetics, Microbiology and Statistics, University of Barcelona, Barcelona, Catalonia, Spain., Coll NS; Centre for Research in Agricultural Genomics (CSIC-IRTA-UAB-UB), Bellaterra, Catalonia, Spain., Orellano E; Área Biología Molecular, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario and Instituto de Biología Molecular y Celular de Rosario, Consejo Nacional de Investigaciones Científicas y Técnicas (IBR-UNR-CONICET), Rosario, Santa Fe, Argentina., Valls M; Department of Genetics, Microbiology and Statistics, University of Barcelona, Barcelona, Catalonia, Spain.; Centre for Research in Agricultural Genomics (CSIC-IRTA-UAB-UB), Bellaterra, Catalonia, Spain.
Jazyk: angličtina
Zdroj: PLoS pathogens [PLoS Pathog] 2023 Dec 19; Vol. 19 (12), pp. e1011888. Date of Electronic Publication: 2023 Dec 19 (Print Publication: 2023).
DOI: 10.1371/journal.ppat.1011888
Abstrakt: Bacterial pathogens exhibit a remarkable ability to persist and thrive in diverse ecological niches. Understanding the mechanisms enabling their transition between habitats is crucial to control dissemination and potential disease outbreaks. Here, we use Ralstonia solanacearum, the causing agent of the bacterial wilt disease, as a model to investigate pathogen adaptation to water and soil, two environments that act as bacterial reservoirs, and compare this information with gene expression in planta. Gene expression in water resembled that observed during late xylem colonization, with an intriguing induction of the type 3 secretion system (T3SS). Alkaline pH and nutrient scarcity-conditions also encountered during late infection stages-were identified as the triggers for this T3SS induction. In the soil environment, R. solanacearum upregulated stress-responses and genes for the use of alternate carbon sources, such as phenylacetate catabolism and the glyoxylate cycle, and downregulated virulence-associated genes. We proved through gain- and loss-of-function experiments that genes associated with the oxidative stress response, such as the regulator OxyR and the catalase KatG, are key for bacterial survival in soil, as their deletion cause a decrease in culturability associated with a premature induction of the viable but non culturable state (VBNC). This work identifies essential factors necessary for R. solanacearum to complete its life cycle and is the first comprehensive gene expression analysis in all environments occupied by a bacterial plant pathogen, providing valuable insights into its biology and adaptation to unexplored habitats.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2023 de Pedro-Jové et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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