Viability and oxidative stress of dental pulp cells after indirect application of chemomechanical agents: An in vitro study.

Autor: Lins-Candeiro CL; Post-graduation Program in Dentistry, School of Dentistry, Federal University of Uberlândia, Uberlândia, Minas Gerais, Brazil., Paranhos LR; Division of Preventive and Community Dentistry, School of Dentistry, Federal University of Uberlândia, Minas Gerais, Brazil., de Oliveira Neto NF; Post-graduation Program in Dentistry, School of Dentistry, Federal University of Uberlândia, Uberlândia, Minas Gerais, Brazil., Ribeiro RAO; Department of Dental Materials and Prosthesis, School of Dentistry, São Paulo State University Júlio de Mesquita Filho, Araraquara, São Paulo, Brazil., de-Souza-Costa CA; Department of Physiology and Pathology, School of Dentistry, São Paulo State University Júlio de Mesquita Filho, Araraquara, São Paulo, Brazil., Guedes FR; Post-graduation Program in Dentistry, School of Dentistry, Federal University of Uberlândia, Uberlândia, Minas Gerais, Brazil., da Silva WHT; Post-graduation Program in Dentistry, School of Dentistry, Federal University of Uberlândia, Uberlândia, Minas Gerais, Brazil., Turrioni AP; Division of Pediatric Dentistry and Orthodontics, School of Dentistry, Federal University of Uberlândia, Uberlândia, Minas Gerais, Brazil., Santos Filho PCF; Division of Restorative Dentistry and Dental Materials, School of Dentistry, Federal University of Uberlândia, Uberlândia, Minas Gerais, Brazil.
Jazyk: angličtina
Zdroj: International endodontic journal [Int Endod J] 2024 Mar; Vol. 57 (3), pp. 315-327. Date of Electronic Publication: 2023 Dec 18.
DOI: 10.1111/iej.14013
Abstrakt: Aim: This study evaluated the transdentinal cytotoxic effects of enzymatic agents (EA) for chemomechanical carious tissue removal on human dental pulp cells.
Methodology: The groups were based on the performed dentine treatments (n = 8): G1: Positive Control (PC - no treatment); G2: Negative Control (NC - 35% H 2 O 2 for 2 min); G3: Brix 3000™ (BX) for 30 s; G4: BX for 2 min; G5: Papacarie Duo™ (PD) for 30 s; G6: PD for 2 min. The cells were evaluated for viability (VB; MTT assay) and production of reactive oxygen species (ROS; DCFH-DA assay) and nitric oxide (NO; Griess reagent). A scanning electron microscope provided morphological chemical analyses and energy-dispersive X-ray spectroscopy. The data were submitted to the one-way anova statistical test complemented by Tukey (p < .05).
Results: Cell viability decreased by 21.1% and 58.4% in G5 and G6, respectively. ROS production in G3 and G4 maintained basal levels but increased by 171.2% and 75.1% in G5 and G6, respectively.
Conclusions: The Brix3000™ enzymatic agent did not cause indirect cytotoxic effects on pulp cells, regardless of the application time. Conversely, Papacarie Duo™ reduced viability and increased ROS production by pulp cells.
(© 2023 British Endodontic Society. Published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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