Molecular detection of Babesia ovis and blood parameters' investigation reveal hematological and biochemical alterations in babesiosis-infected Lohi sheep in Multan, Pakistan.

Autor: Sajid M; Zoology Division, Institute of Pure and Applied Biology, Bahauddin Zakariya University, Multan, Pakistan., Naqvi SAH; Department of Plant Pathology, Faculty of Agricultural Sciences and Technology, Bahauddin Zakariya University, Multan, Pakistan., Riaz M; Zoology Division, Institute of Pure and Applied Biology, Bahauddin Zakariya University, Multan, Pakistan., Umar UUD; Department of Plant Pathology, Faculty of Agricultural Sciences and Technology, Bahauddin Zakariya University, Multan, Pakistan., Nasreen N; Department of Zoology, Khyber Pakhtunkhwa, Abdul Wali Khan University, Mardan, Pakistan., Khan A; Department of Zoology, Bacha Khan University, Charsadda, Pakistan.; Department of Biology, Mount Allison University, Sackville, Canada., Said MB; Laboratory of Microbiology, National School of Veterinary Medicine of Sidi Thabet, University of Manouba, Manouba, Tunisia.; Department of Basic Sciences, Higher Institute of Biotechnology of Sidi Thabet, University of Manouba, Manouba, Tunisia.
Jazyk: angličtina
Zdroj: Open veterinary journal [Open Vet J] 2023 Nov; Vol. 13 (11), pp. 1400-1408. Date of Electronic Publication: 2023 Nov 30.
DOI: 10.5455/OVJ.2023.v13.i11.2
Abstrakt: Background: Babesia infections in sheep can cause a wide range of clinical and laboratory presentations. Changes in blood parameters are a meaningful manifestation of physiological and pathological changes in an organism.
Aim: Therefore, the present study was conducted to analyze and compare hematological and biochemical parameters between blood profiles of Lohi sheep naturally infected and uninfected with Babesia ovis , the main causative agent of ovine babesiosis.
Methods: Initially, blood and serum samples from 67 Lohi sheep were collected, DNA was extracted and babesial infection was detected through polymerase chain reaction. The overall infection rate of B. ovis was 37% (25/67). Sixteen infected (experiment group) and 16 uninfected (control group) sheep that were apparently healthy with no history of previous treatment for babesiosis, were selected for hemato-biochemical analysis. Blood samples were analyzed through an automatic CBC analyzer, while serum collected from gel vacutainers was analyzed for blood urea, blood urea nitrogen (BUN), creatinine, and total bilirubin. Each parameter was compared between infected and uninfected animals using a paired t -test in Minitab Express™ software for statistical analyses.
Results: Erythron comparison showed a highly significant ( p < 0.0001) decrease in RBC, hemoglobin, and Hct. A nonsignificant increase in mean corpuscular volume (MCV), red cell distribution width (RDW), and RDW-standard deviation (RDW-SD), while a nonsignificant decrease in mean corpuscular haemoglobin (MCH) and MCH concentration (MCHC) values was recorded in infected sheep. Leukon comparison showed a significantly low level of total leukocyte ( p < 0.001) in infected sheep. Platelet (Plt) along with platelet crit (Pct) and platelet distribution width (PDW) were nonsignificantly higher, whereas a nonsignificant decrease in mean Plt volume was recorded in infected sheep as compared to uninfected animals. Among biochemical parameters, blood urea, BUN, and total bilirubin showed significant differences ( p < 0.05), while creatinine showed a nonsignificant difference.
Conclusion: To the best of our knowledge, this is the first report on hemato-biochemical changes associated with babesiosis in the Lohi breed. Consistent with hemolytic anemia, these data would justify physical examination and, together with the medical history, would provide an excellent basis for the diagnosis of babesiosis.
Competing Interests: The authors declare no conflict of interest.
Databáze: MEDLINE
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