Antitumoral properties of the pomegranate peel and blueberry extracts against tongue carcinoma ( in vitro study).

Autor:	Ahmed RM; Oral Pathology Department, Faculty of Dentistry, Mansoura University, Egypt., Esmaeil DAM; Oral Pathology Department, Faculty of Dentistry, Mansoura University, Egypt.; Oral Pathology Department, Sini University, Kantra Campus, Egypt., El-Nagdy SY; Oral Pathology Department, Faculty of Dentistry, Mansoura University, Egypt.; Oral Pathology Department, Faculty of Dentistry, Horus University, Egypt., El-Sisi NA; Oral Pathology Department, Faculty of Dentistry, Mansoura University, Egypt.
Jazyk:	angličtina
Zdroj:	The Saudi dental journal [Saudi Dent J] 2023 Dec; Vol. 35 (8), pp. 985-995. Date of Electronic Publication: 2023 Aug 05.
DOI:	10.1016/j.sdentj.2023.07.021
Abstrakt:	Background: Oral squamous cell carcinoma (OSCC) is one of the most common cancers globally. Considering the drawbacks of the traditional treatment phytochemicals have been introduced to the research field with consideration of their minimal, or no side effects and good efficacy against cancer cells. Pomegranate peel and blueberries are very well-known phytochemicals in this context. Material and Methods: Methanolic extracts are prepared from both pomegranate peel (PPE) and blueberry (BE) dried powders. The antioxidant content was determined by ORAC assay for both extracts. The cytotoxic levels of the extracts and IC50 on the HNO-97 cell line were defined using the colorimetric SRB assay. Then flow cytometric apoptosis analysis of the IC50 of both extracts was performed. Finally, an investigation of the metastasis through the wound healing assay was performed for both extracts. Results: A significant difference in the antioxidant content was found between PPE and BE ethanolic extracts. The Ic50 for the PPE-treated cell line was 73.35 µg/ml while the BE showed it at 525.38 µg/ml with a significant difference between them. Both PPE and BE showed significant induction of cancer cell apoptosis with much better results with PPE treatment. The wound healing assay showed significant inhibition of cell migration when treated with PPE while there was not any significant effect on cell migration when treated with BE. Conclusion: With the consideration that, the Phytochemicals used are well-known fruits with no harm on normal tissues. Low doses of PPE exert incredibly significant alteration in the HNO-97 tongue cancer cell proliferation, inducing apoptosis and inhibition of cancer cell migration. Meanwhile, treatment with BE needs much higher doses for showing anti-proliferative properties of cancer cells and induce cancer cell apoptosis. This gives promising results for further investigations about using them as a treatment or adjunctive treatment for oral cancer cases. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2023 The Authors.)
Databáze:	MEDLINE
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