RpoN (sigma factor 54) contributes to bacterial fitness during tracheal colonization of Bordetella bronchiseptica.

Autor: Ma X; Department of Molecular Bacteriology, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan., Nugraha DK; Department of Molecular Bacteriology, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan., Hiramatsu Y; Department of Molecular Bacteriology, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan., Horiguchi Y; Department of Molecular Bacteriology, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.; Center for Infectious Disease Education and Research, Osaka University, Osaka, Japan.
Jazyk: angličtina
Zdroj: Microbiology and immunology [Microbiol Immunol] 2024 Feb; Vol. 68 (2), pp. 36-46. Date of Electronic Publication: 2023 Dec 17.
DOI: 10.1111/1348-0421.13109
Abstrakt: The Gram-negative pathogenic bacterium Bordetella bronchiseptica is a respiratory pathogen closely related to Bordetella pertussis, the causative agent of whooping cough. Despite sharing homologous virulence factors, B. bronchiseptica infects a broad range of mammalian hosts, including some experimental animals, whereas B. pertussis is strictly adapted to humans. Therefore, B. bronchiseptica is often used as a representative model to explore the pathogenicity of Bordetella in infection experiments with laboratory animals. Although Bordetella virulence factors, including toxins and adhesins have been studied well, our recent study implied that unknown virulence factors are involved in tracheal colonization and infection. Here, we investigated bacterial genes contributing to tracheal colonization by high-throughput transposon sequencing (Tn-seq). After the screening, we picked up 151 candidate genes of various functions and found that a rpoN-deficient mutant strain was defective in tracheal colonization when co-inoculated with the wild-type strain. rpoN encodes σ 54 , a sigma factor that regulates the transcription of various genes, implying its contribution to various bacterial activities. In fact, we found RpoN of B. bronchiseptica is involved in bacterial motility and initial biofilm formation. From these results, we propose that RpoN supports bacterial colonization by regulating various bacteriological functions.
(© 2023 The Authors. Microbiology and Immunology published by The Societies and John Wiley & Sons Australia, Ltd.)
Databáze: MEDLINE
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