Validation of a multiplex-tandem RT-PCR for the detection of bovine respiratory disease complex using Scottish bovine lung samples.

Autor: Fergusson M; SRUC Veterinary and Analytical Services, Pentland Science Park, Bush Loan Road, Edinburgh, Scotland EH26 0PZ, UK., Maley M; Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik, Scotland EH26 0PZ, UK., Geraghty T; SRUC Veterinary and Analytical Services, Pentland Science Park, Bush Loan Road, Edinburgh, Scotland EH26 0PZ, UK., Albaladejo JP; Department of Computer Sciences, Applied Mathematics and Statistics, University of Girona, Girona 17003, Spain; Biomathematics and Statistics Scotland, The King's Buildings, Peter Guthrie Tait Road, Edinburgh, Scotland EH9 3FD, UK., Mason C; SRUC Veterinary and Analytical Services, Pentland Science Park, Bush Loan Road, Edinburgh, Scotland EH26 0PZ, UK., Rocchi MS; Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik, Scotland EH26 0PZ, UK. Electronic address: mara.rocchi@moredun.ac.uk.
Jazyk: angličtina
Zdroj: Veterinary journal (London, England : 1997) [Vet J] 2024 Feb; Vol. 303, pp. 106058. Date of Electronic Publication: 2023 Dec 14.
DOI: 10.1016/j.tvjl.2023.106058
Abstrakt: The welfare and economic impact of bovine respiratory disease complex (BRDC), and its associated antibiotic usage, are major challenges to cattle rearing and beef cattle finishing industries. Accurate pathogen diagnosis is important to undertake appropriate treatment and long-term management strategies, such as vaccine selection. Conventional diagnostic approaches have several limitations including high costs, long turnaround times and difficulty in test interpretation, which could delay treatment decisions and lead to unnecessary animal losses. We describe the validation of a multiplex-tandem (MT) reverse transcription-polymerase chain reaction (RT-PCR) for the detection of seven common pathogens associated with BRDC. This test has the potential to advance pathogen identification and to overcome many of the limitations of current testing methods. It requires a single sample and results are obtained quickly and not influenced by prior antimicrobial therapy or overgrowth of contaminating organisms. We demonstrated a test specificity of 100% and sensitivity ranging from 93.5% to 100% for these seven common pathogens. This test will be a useful addition to advance BRDC investigation and diagnosis.
Competing Interests: Declaration of Competing Interest None of the authors has any financial or personal relationships that could inappropriately influence or bias the content of the paper. Ausdiagnostic played no role in the above study design nor in the collection, analysis and interpretation of data of the field validation, or in the decision to submit the manuscript for publication.
(Copyright © 2023 The Authors. Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
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