Comparison of one novel and four established diagnostic tests for insulin dysregulation in ponies.

Autor: Carslake HB; Institute of Infection, Veterinary and Ecological sciences, Faculty of Health and Life Sciences, University of Liverpool, Leahurst, UK CH64 7TE, UK. Electronic address: hbc@liverpool.ac.uk., Pinchbeck GL; Institute of Infection, Veterinary and Ecological sciences, Faculty of Health and Life Sciences, University of Liverpool, Leahurst, UK CH64 7TE, UK., Argo CM; Scotland's Rural College, Aberdeen, Scotland AB21 9YA, UK., Dugdale AHA; Paragon Veterinary referrals, Wakefield UK WF1 2DF, UK., McGowan CM; Institute of Infection, Veterinary and Ecological sciences, Faculty of Health and Life Sciences, University of Liverpool, Leahurst, UK CH64 7TE, UK.
Jazyk: angličtina
Zdroj: Veterinary journal (London, England : 1997) [Vet J] 2024 Feb; Vol. 303, pp. 106059. Date of Electronic Publication: 2023 Dec 14.
DOI: 10.1016/j.tvjl.2023.106059
Abstrakt: Several tests have been advocated for diagnosis of insulin dysregulation (ID). Tests using simple sugars may not reflect the response to naturally ingested carbohydrates. This study aimed to evaluate agreement between the oral glucose test (OGT), the oral sugar test (OST), a novel oral test using a proprietary cereal (WEET), the IV combined glucose-insulin tolerance test (CGIT) and fasted basal insulin (FI) for diagnosis of ID. Each of the five tests above was performed on a group of six normal and six insulin dysregulated mixed-breed ponies in a randomised crossover study. Area under the curve (AUC) and maximum concentration of insulin from OGT, OST and WEET showed strong to very strong bivariate correlations (r = 0.85-0.94, and r = 0.87-0.92, respectively; P ≤ 0.001) and were significantly different between tests (associated with dose of carbohydrate) and between CGIT-positive and -negative ponies. Dichotomous results showed substantial agreement between OST and both WEET (κ = 0.65; P = 0.02) and OGT (κ = 0.67; P = 0.01) and between CGIT and both OST (κ = 0.63; P = 0.03) and OGT (κ = 0.67; P = 0.01), and no agreement between FI, which had low sensitivity, and all other tests (κ = 0.15 - 0.31; P > 0. 05). Palatability of WEET was variable, resulting in one pony being excluded for analysis of WEET data. Further work on development of an oral test using a more palatable feedstuff and appropriate cut-offs or diagnostic thresholds for tests of ID is warranted.
Competing Interests: Declaration of Competing Interest None of the authors has a financial or personal relationship with other people or organisations that could inappropriately influence or via the content of the paper.
(Copyright © 2023 The Authors. Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
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