Self-Degrading Multifunctional PEG-Based Hydrogels-Tailormade Substrates for Cell Culture.

Autor: Kowalczuk K; Institute of Organic Chemistry and Macromolecular Chemistry (IOMC), Friedrich-Schiller-University Jena, Lessingstraße 8, 07743, Jena, Germany.; Jena Center for Soft Matter (JCSM), Friedrich-Schiller-University Jena, Philosophenweg 7, 07743, Jena, Germany.; Cluster of Excellence Balance of the Microverse, Friedrich Schiller University Jena, Grüne Aue, D-07754, Jena, Germany., Dasgupta A; Leibniz Institute of Photonic Technology, Albert-Einstein-Straße 9, 07745, Jena, Germany.; Institute of Applied Optics and Biophysics, Friedrich-Schiller-University Jena, Helmholtzweg 4, 07743, Jena, Germany., Páez Larios F; Leibniz Institute of Photonic Technology, Albert-Einstein-Straße 9, 07745, Jena, Germany.; Institute of Applied Optics and Biophysics, Friedrich-Schiller-University Jena, Helmholtzweg 4, 07743, Jena, Germany., Ulrich HF; Institute of Organic Chemistry and Macromolecular Chemistry (IOMC), Friedrich-Schiller-University Jena, Lessingstraße 8, 07743, Jena, Germany.; Jena Center for Soft Matter (JCSM), Friedrich-Schiller-University Jena, Philosophenweg 7, 07743, Jena, Germany., Wegner V; Institute of Biochemistry II, Jena University Hospital, Am Nonnenplan 2-4, 07743, Jena, Germany., Brendel JC; Institute of Organic Chemistry and Macromolecular Chemistry (IOMC), Friedrich-Schiller-University Jena, Lessingstraße 8, 07743, Jena, Germany.; Jena Center for Soft Matter (JCSM), Friedrich-Schiller-University Jena, Philosophenweg 7, 07743, Jena, Germany., Eggeling C; Jena Center for Soft Matter (JCSM), Friedrich-Schiller-University Jena, Philosophenweg 7, 07743, Jena, Germany.; Cluster of Excellence Balance of the Microverse, Friedrich Schiller University Jena, Grüne Aue, D-07754, Jena, Germany.; Leibniz Institute of Photonic Technology, Albert-Einstein-Straße 9, 07745, Jena, Germany.; Institute of Applied Optics and Biophysics, Friedrich-Schiller-University Jena, Helmholtzweg 4, 07743, Jena, Germany., Mosig AS; Cluster of Excellence Balance of the Microverse, Friedrich Schiller University Jena, Grüne Aue, D-07754, Jena, Germany.; Institute of Biochemistry II, Jena University Hospital, Am Nonnenplan 2-4, 07743, Jena, Germany.; Center for Sepsis Control and Care, Jena University Hospital, Am Klinikum 1, 07747, Jena, Germany., Schacher FH; Institute of Organic Chemistry and Macromolecular Chemistry (IOMC), Friedrich-Schiller-University Jena, Lessingstraße 8, 07743, Jena, Germany.; Jena Center for Soft Matter (JCSM), Friedrich-Schiller-University Jena, Philosophenweg 7, 07743, Jena, Germany.; Cluster of Excellence Balance of the Microverse, Friedrich Schiller University Jena, Grüne Aue, D-07754, Jena, Germany.
Jazyk: angličtina
Zdroj: Macromolecular bioscience [Macromol Biosci] 2024 May; Vol. 24 (5), pp. e2300383. Date of Electronic Publication: 2024 Jan 02.
DOI: 10.1002/mabi.202300383
Abstrakt: The use of PEG-based hydrogels as cell culture matrix to mimic the natural extracellular matrix (ECM) has been realized using a range of well-defined, tunable, and dynamic scaffolds, although they require cell adhesion ligands such as RGDS-peptide (Arg-Gly-Asp-Ser) to promote cell adhesion. Herein the synthesis of ionic and degradable hydrogels is demonstrated for cell culture by crosslinking [PEG-SH] 4 with the zwitterionic crosslinker N,N-bis(acryloxyethyl)-N-methyl-N-(3-sulfopropyl) ammonium betaine (BMSAB) and the cationic crosslinker N,N-bis(acryloxyethyl)-N,N-dimethyl-1-ammonium iodide (BDMAI). Depending on the amount of ionic crosslinker used in gel formation, the hydrogels show tunable gelation time and stiffness. At the same time, the ionic groups act as catalysts for hydrolytic degradation, thereby allowing to define a stability window. The latter could be tailored in a straightforward manner by introducing the non-degradable crosslinker tri(ethylene glycol) divinyl ether. In addition, both ionic crosslinkers favor cell attachment in comparison to the pristine PEG hydrogels. The degradation is examined by swelling behavior, rheology, and fluorescence correlation spectroscopy indicating degradation kinetics depending on diffusion of incorporated fluorescent molecules.
(© 2024 The Authors. Macromolecular Bioscience published by Wiley‐VCH GmbH.)
Databáze: MEDLINE
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