Electrotransfer for nucleic acid and protein delivery.

Autor: Muralidharan A; Department of Bionanoscience, Delft University of Technology, van der Maasweg 9, 2629 HZ Delft, The Netherlands; Kavli Institute of Nanoscience, Delft University of Technology, van der Maasweg 9, 2629 HZ Delft, The Netherlands. Electronic address: a.muralidharan@tudelft.nl., Boukany PE; Department of Chemical Engineering, Delft University of Technology, van der Maasweg 9, 2629 HZ Delft, The Netherlands. Electronic address: p.e.boukany@tudelft.nl.
Jazyk: angličtina
Zdroj: Trends in biotechnology [Trends Biotechnol] 2024 Jun; Vol. 42 (6), pp. 780-798. Date of Electronic Publication: 2023 Dec 14.
DOI: 10.1016/j.tibtech.2023.11.009
Abstrakt: Electrotransfer of nucleic acids and proteins has become crucial in biotechnology for gene augmentation and genome editing. This review explores the applications of electrotransfer in both ex vivo and in vivo scenarios, emphasizing biomedical uses. We provide insights into completed clinical trials and successful instances of nucleic acid and protein electrotransfer into therapeutically relevant cells such as immune cells and stem and progenitor cells. In addition, we delve into emerging areas of electrotransfer where nanotechnology and deep learning techniques overcome the limitations of traditional electroporation.
Competing Interests: Declaration of interests The authors declare no conflicts of interest.
(Copyright © 2023 The Author(s). Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
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