Comparative analysis of diagnostic assays for scrub typhus: Unveiling enhanced approaches for accurate detection.

Autor: Barbuddhe SS; Maharashtra Animal and Fishery Sciences University, Nagpur 440006, India., Thorat YT; Maharashtra Animal and Fishery Sciences University, Nagpur 440006, India., Kulkarni P; Maharashtra Animal and Fishery Sciences University, Nagpur 440006, India., Shinde SV; Maharashtra Animal and Fishery Sciences University, Nagpur 440006, India., Chaudhari SP; Maharashtra Animal and Fishery Sciences University, Nagpur 440006, India., Kurkure NV; Maharashtra Animal and Fishery Sciences University, Nagpur 440006, India., Sahu R; Odisha University of Agriculture and Technology, Bhubaneswar 751003, India., Rawool DB; ICAR-National Meat Research Institute, Hyderabad 500092, India. Electronic address: Deepak.rawool@yahoo.com.
Jazyk: angličtina
Zdroj: Journal of microbiological methods [J Microbiol Methods] 2024 Jan; Vol. 216, pp. 106875. Date of Electronic Publication: 2023 Dec 13.
DOI: 10.1016/j.mimet.2023.106875
Abstrakt: The study comparatively evaluated serological assays, namely, Weil Felix assay, and IgM ELISA with the gold-standard immunofluorescence test (IFAT) for the sensitive and specific serodiagnosis of scrub typhus infection in occupationally exposed groups of humans. A total of 78 serum samples collected from persons affected with various ailments and belonging to different risk groups were screened in the study. Out of the 78 serum samples tested, a total of 17, 26, and 47 samples turned out to be positive by IFAT, IgM ELISA, and Weil Felix test, respectively. The Weil Felix assay could not serve as an ideal test for screening scrub typhus infection owing to its poor sensitivity and specificity in comparison with IFAT. IgM-ELISA could be an initial screening test to detect scrub typhus suspected patient in limited resource settings.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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