Establishment of oral microbiome in very low birth weight infants during the first weeks of life and the impact of oral diet implementation.

Autor:	Vanzele PAR; Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, University of São Paulo, São Paulo, SP, Brazil., Sparvoli LG; Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, University of São Paulo, São Paulo, SP, Brazil., de Camargo PP; Neonatal Intensive Care Center, Children's Institute, Hospital das Clínicas, São Paulo Medical School, University of São Paulo, São Paulo, SP, Brazil., Tragante CR; Neonatal Intensive Care Center, Children's Institute, Hospital das Clínicas, São Paulo Medical School, University of São Paulo, São Paulo, SP, Brazil., Beozzo GPNS; Neonatal Intensive Care Center, Children's Institute, Hospital das Clínicas, São Paulo Medical School, University of São Paulo, São Paulo, SP, Brazil., Krebs VLJ; Neonatal Intensive Care Center, Children's Institute, Hospital das Clínicas, São Paulo Medical School, University of São Paulo, São Paulo, SP, Brazil., Cortez RV; Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, University of São Paulo, São Paulo, SP, Brazil., Taddei CR; Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences, University of São Paulo, São Paulo, SP, Brazil.; School of Arts, Sciences and Humanity, University of São Paulo, São Paulo, SP, Brazil.; Division of Clinical Laboratory, University Hospital - University of São Paulo, São Paulo, SP, Brazil.
Jazyk:	angličtina
Zdroj:	PloS one [PLoS One] 2023 Dec 15; Vol. 18 (12), pp. e0295962. Date of Electronic Publication: 2023 Dec 15 (Print Publication: 2023).
DOI:	10.1371/journal.pone.0295962
Abstrakt:	Very low birth weight (VLBW) infants, mostly preterm, have many barriers to feeding directly from the mother's breast, and need to be fed alternatively. Feeding is a major influencer in oral microbial colonization, and this colonization in early life is crucial for the promotion of human health. Therefore, this research aimed to observe the establishment of oral microbiome in VLBW infants during their first month of life through hospitalization, and to verify the impact caused by the implementation of oral diet on the colonization of these newborns. We included 23 newborns followed during hospitalization and analyzed saliva samples collected weekly, using 16S rRNA gene sequencing. We observed a significant decrease in richness and diversity and an increase in dominance over time (q-value < 0.05). The oral microbiome is highly dynamic during the first weeks of life, and beta diversity suggests a microbial succession in early life. The introduction of oral diet does not change the community structure, but affects the abundance, especially of Streptococcus. Our results indicate that although time is related to significant changes in the oral microbial profile, oral feeding benefits genera that will remain colonizers throughout the host's life. Competing Interests: The authors have declared that no competing interests exist. (Copyright: © 2023 Vanzele et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze:	MEDLINE
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