Ultrasonic Electroporation for Cells Grown on Piezoelectric Film Composed of Hydroxyapatite Nanowire and PVDF.

Autor: Dolai J; School of Materials Science, Indian Association for the Cultivation of Science, 2A & 2B Raja S. C. Mullick Road, Kolkata 700032, India., Sarkar AR; School of Materials Science, Indian Association for the Cultivation of Science, 2A & 2B Raja S. C. Mullick Road, Kolkata 700032, India., Maity A; School of Materials Science, Indian Association for the Cultivation of Science, 2A & 2B Raja S. C. Mullick Road, Kolkata 700032, India., Mukherjee B; School of Materials Science, Indian Association for the Cultivation of Science, 2A & 2B Raja S. C. Mullick Road, Kolkata 700032, India., Jana NR; School of Materials Science, Indian Association for the Cultivation of Science, 2A & 2B Raja S. C. Mullick Road, Kolkata 700032, India.
Jazyk: angličtina
Zdroj: ACS applied materials & interfaces [ACS Appl Mater Interfaces] 2023 Dec 27; Vol. 15 (51), pp. 59155-59164. Date of Electronic Publication: 2023 Dec 15.
DOI: 10.1021/acsami.3c13005
Abstrakt: The delivery of cell impermeable exogenous material into live cells by external stimuli is critical for both biological research and therapeutic applications. Although electroporation-based delivery of foreign materials inside the cell is a powerful approach, cell viability is often compromised due to the requirement of high voltage. Here, we report a piezoelectric hydroxyapatite nanowire-embedded poly(vinylidene fluoride) (PVDF) film for ultrasonic electroporation-based delivery of foreign materials to adherent cells. We found that 9 wt % loading of hydroxyapatite nanowires into PVDF can enhance the piezoelectric property by 2-3 times (with a piezoelectric constant value of 58 pm/V) than pure PVDF/nanowire, which is comparable to commonly known piezoelectric ceramics. These films can harvest mechanical as well as ultrasound-based energy to produce electrical potential up to 2 V. This biocompatible film can be used to grow cells on top of it and for subsequent application of ultrasound to exert electric voltage on cell membrane. We found that ultrasonic exposure to adhered cells leads to reversible pore formation on cell membrane that offers intracellular delivery of FITC-dextran with 75% efficiency. The developed piezoelectric film-based ultrasonic electroporation can be used for wireless electroporation in remote areas.
Databáze: MEDLINE
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