Epithelial Immune Cell Response to Initial Soft Contact Lens Wear in the Human Corneal and Conjunctival Epithelium.

Autor:	Mobeen R; School of Optometry and Vision Science, University of New South Wales, New South Wales, Sydney, Australia., Stapleton F; School of Optometry and Vision Science, University of New South Wales, New South Wales, Sydney, Australia., Chao C; School of Optometry and Vision Science, University of New South Wales, New South Wales, Sydney, Australia., Huynh MC; School of Optometry and Vision Science, University of New South Wales, New South Wales, Sydney, Australia., Phoebe Wong YS; School of Optometry and Vision Science, University of New South Wales, New South Wales, Sydney, Australia., Naduvilath T; School of Optometry and Vision Science, University of New South Wales, New South Wales, Sydney, Australia.; Brien Holden Vision Institute, University of New South Wales, New South Wales, Sydney, Australia., Golebiowski B; School of Optometry and Vision Science, University of New South Wales, New South Wales, Sydney, Australia.
Jazyk:	angličtina
Zdroj:	Investigative ophthalmology & visual science [Invest Ophthalmol Vis Sci] 2023 Dec 01; Vol. 64 (15), pp. 18.
DOI:	10.1167/iovs.64.15.18
Abstrakt:	Purpose: The purpose of this study was to assess the immediate ocular immune response to soft contact lens (CL) wear by examining presumed epithelial immune cell (EIC) density and morphology at the central, peripheral, limbal cornea, and conjunctiva. Methods: Fifty-four participants naïve to CL wear (mean age = 24.8 ± 9.8 years, 44% female participants), were examined using in vivo confocal microscopy at baseline and after 2 hours of CL wear (1-Day ACUVUE MOIST). Images were captured at the central, temporal far peripheral and limbal cornea, and bulbar conjunctiva. EIC density was counted manually and morphology was graded. Differences in EIC parameters pre- and post-CL wear were examined using a generalized estimating equation model with appropriate post hoc analyses. Results: After 2 hours of soft CL wear, there was a significant increase in EIC density in all regions other than the central cornea (all P < 0.001). Cell body size was significantly larger, and a higher proportion of participants exhibited EIC with long dendrites after lens wear at the central and peripheral cornea (both P < 0.001). There was a significant increase in the number of participants displaying EIC with thick dendrites at the peripheral (P = 0.04) and limbal cornea (P < 0.001) after lens wear. Conclusions: EICs were primarily recruited to the peripheral regions, whereas the central cornea shows no significant recruitment after short-term CL wear. Both central and peripheral corneas exhibited an enhanced antigen capture capacity, whereas migratory capacity was increased in the peripheral corneal regions suggesting EIC activation following a short period of CL wear.
Databáze:	MEDLINE
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