Valorization of the essential oil from Drypetes gossweileri S. Moore (Putranjivaceae): in vitro, in vivo , and in silico nematicidal activity.

Autor: Mbula JP; Faculté des Sciences, Université de Kisangani, Kisangani, Democratic Republic of Congo., Andres MF; Instituto de Ciencias Agrarias, Consejo Superior de Investigaciones Científicas, Madrid, Spain., Kitete EM; Faculté des Sciences, Université de Kinshasa, Kinshasa, Democratic Republic of Congo., Kasiama NG; Faculté des Sciences, Université de Kinshasa, Kinshasa, Democratic Republic of Congo., Tshilanda DD; Faculté des Sciences, Université de Kinshasa, Kinshasa, Democratic Republic of Congo., Ngbolua KN; Faculté des Sciences, Université de Kinshasa, Kinshasa, Democratic Republic of Congo., Tshibangu DST; Faculté des Sciences, Université de Kinshasa, Kinshasa, Democratic Republic of Congo., Onautshu O; Faculté des Sciences, Université de Kisangani, Kisangani, Democratic Republic of Congo., González-Coloma A; Instituto de Ciencias Agrarias, Consejo Superior de Investigaciones Científicas, Madrid, Spain., Mpiana PT; Faculté des Sciences, Université de Kinshasa, Kinshasa, Democratic Republic of Congo.
Jazyk: angličtina
Zdroj: Frontiers in plant science [Front Plant Sci] 2023 Nov 29; Vol. 14, pp. 1260360. Date of Electronic Publication: 2023 Nov 29 (Print Publication: 2023).
DOI: 10.3389/fpls.2023.1260360
Abstrakt: The chemical composition, insect antifeedant, in vtro/in vivo nematicidal activity, phytotoxicity, and in silico nematicidal activity of the essential oil (EO) of the African medicinal plant Drypetes gossweileri were studied. Chemical analysis using GC/MS indicated that benzyl isothiocyanate (96.23%) was the major compound, followed by benzyl cyanide (1.38%). The biocidal effects of this oil were tested against insect pests and root-knot nematodes. All the insect species tested were significantly affected by the oil according to their feeding adaptations ( Spodoptera littoralis and Myzus persicae were less affected than Rhopalosiphum padi ) with efficient doses (EC 50 ) of 29.4 8.3 μg/cm 2 , 14.744 8.3 μg/cm 2 , and 8.3 μg/cm 2 , respectively. The oil was highly effective against juveniles J2 of the nematode Meloidogyne javanica , with LC 50 -LC 90 values of 0.007 mg/mL-0.0113 mg/mL. D. gossweileri EO at minimum lethal concentrations (MLC) and below strongly inhibited egg hatching in vitro , whereas soil treatment caused a strong suppression of nematode population, infection frequency, and multiplication rate. The EO inhibited ryegrass ( Lolium perenne ) germination at 0.4 mg/mL, while at 0.1 mg/mL, its effects on germination, root and leaf growth were moderate (32.4%, 8.4%, and 18.3%, respectively). The tomato ( Solanum lycopersicum ) germination was not affected by the EO, but the root growth was reduced (56% at 0.1 mg/mL) at a dose 10 times higher than the LD 50 calculated for M. javanica J2 mortality. Molecular docking of the nematicidal effects of the oil using PyRx revealed a strong interaction between potassium chloride transporting KCC3 (PDB ID: 7D90) and benzyl cyanide at a distance of 2.20 A° with GLN C:350, followed by benzyl isothiocyanate at a distance of 2.78 A° with ARG B:294. The in vivo nematicidal effects of D. gossweileri EO on M. javanica penetration and reproduction in tomato roots further support the potential of this EO as a nematicidal agent with insect antifeedant effects, which could be used by local farmers for crop protection.
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2023 Mbula, Andres, Kitete, Kasiama, Tshilanda, Ngbolua, Tshibangu, Onautshu, González-Coloma and Mpiana.)
Databáze: MEDLINE
Externí odkaz: