Production and characterization of polyhydroxyalkanoates by Halomonas alkaliantarctica utilizing dairy waste as feedstock.

Autor: Mozejko-Ciesielska J; Department of Microbiology and Mycology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10719, Olsztyn, Poland. justyna.mozejko@uwm.edu.pl., Moraczewski K; Institute of Materials Engineering, Kazimierz Wielki University, 85064, Bydgoszcz, Poland., Czaplicki S; Department of Plant Food Chemistry and Processing, Faculty of Food Science, University of Warmia and Mazury in Olsztyn, Pl. Cieszyński 1, 10726, Olsztyn, Poland., Singh V; Department of Biosciences, School of Science, Indrashil University, Rajpur, Mehsana, 382715, India.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2023 Dec 15; Vol. 13 (1), pp. 22289. Date of Electronic Publication: 2023 Dec 15.
DOI: 10.1038/s41598-023-47489-8
Abstrakt: Currently, the global demand for polyhydroxyalkanoates (PHAs) is significantly increasing. PHAs are produced by several bacteria that are an alternative source of synthetic polymers derived from petrochemical refineries. This study established a simple and more feasible process of PHA production by Halomonas alkaliantarctica using dairy waste as the only carbon source. The data confirmed that the analyzed halophile could metabolize cheese whey (CW) and cheese whey mother liquor (CWML) into biopolyesters. The highest yield of PHAs was 0.42 g/L in the cultivation supplemented with CWML. Furthermore, it was proved that PHA structure depended on the type of by-product from cheese manufacturing, its concentration, and the culture time. The results revealed that H. alkaliantarctica could produce P(3HB-co-3HV) copolymer in the cultivations with CW at 48 h and 72 h without adding of any precursors. Based on the data obtained from physicochemical and thermal analyses, the extracted copolymer was reported to have properties suitable for various applications. Overall, this study described a promising approach for valorizing of dairy waste as a future strategy of industrial waste management to produce high value microbial biopolymers.
(© 2023. The Author(s).)
Databáze: MEDLINE
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