| Autor: |
Kim S; Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju-gun, Jeollabuk-do 55365, Republic of Korea., Heo J; Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju-gun, Jeollabuk-do 55365, Republic of Korea., Kwon SW; Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju-gun, Jeollabuk-do 55365, Republic of Korea., Lee D; Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju-gun, Jeollabuk-do 55365, Republic of Korea., Han BH; Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju-gun, Jeollabuk-do 55365, Republic of Korea., Hong SB; Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju-gun, Jeollabuk-do 55365, Republic of Korea., Kim Y; Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju-gun, Jeollabuk-do 55365, Republic of Korea. |
| Abstrakt: |
An aerobic, Gram-stain-positive, rod-shaped, endospore-forming bacterial strain, designated BB3-R1 T , was isolated from cow faeces sampled in Daejeon, Republic of Korea. Growth was observed at 25-45 °C (optimum, 35-40 °C) and pH 7.0-9.0 (optimum, pH 8.0), with up to 3 % (w/v) NaCl (optimum, 0 % NaCl). blast analysis of 16S rRNA gene sequences revealed the highest sequence similarity of strain BB3-R1 T to Brevibacillus borstelensis NRRL NRS-818 T (98.8 %) followed by Brevibacillus panacihumi JCM 15085 T (97.5 %). According to 16S rRNA gene and whole-genome based phylogenetic trees, strain BB3-R1 T clustered with Brevibacillus composti FJAT-54423 T and B. borstelensis NRRL NRS-818 T . OrthoANI and dDDH values of strain BB3-R1 T with the closely related strains were lower than 77.5 and 26.8 %, respectively. The major menaquinones and polar lipids of the strain were MK-7 and phosphatidylmonomethylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, respectively. The major fatty acids (>10 %) were C 14 : 0 iso, C 15 : 0 iso, C 15 : 0 anteiso and C 16 : 1 ω 7 c alcohol. The cell-wall peptidoglycan contained cross-linked meso -diaminopimelic acid (type A1 gamma). The phenotypic, chemotaxonomic and genotypic data obtained in this study showed that the strain represents a novel species of the genus Brevibacillus , for which the name Brevibacillus ruminantium sp. nov. (type strain BB3-R1 T =KACC 22663 T =NBRC 115962 T ) is proposed. |
