Cytoplasmic Accumulation and Permeability of Antibiotics in Gram Positive and Gram Negative Bacteria Visualized in Real-Time via a Fluorogenic Tagging Strategy.

Autor:	Squire SO; Department of Chemistry, University of Utah, Salt Lake City, Utah 84112, United States.; Henry Eyring Center for Cell & Genome Science, University of Utah, Salt Lake City, Utah 84112, United States., Sebghati S; Department of Chemistry, University of Utah, Salt Lake City, Utah 84112, United States.; Henry Eyring Center for Cell & Genome Science, University of Utah, Salt Lake City, Utah 84112, United States., Hammond MC; Department of Chemistry, University of Utah, Salt Lake City, Utah 84112, United States.; Henry Eyring Center for Cell & Genome Science, University of Utah, Salt Lake City, Utah 84112, United States.
Jazyk:	angličtina
Zdroj:	ACS chemical biology [ACS Chem Biol] 2024 Jan 19; Vol. 19 (1), pp. 3-8. Date of Electronic Publication: 2023 Dec 14.
DOI:	10.1021/acschembio.3c00510
Abstrakt:	In this study, we describe the first real-time live cell assay for compound accumulation and permeability in both Gram positive and Gram negative bacteria. The assay utilizes a novel fluorogenic tagging strategy that permits direct visualization of compound accumulation dynamics in the cytoplasm of live cells, unobscured by washing or other processing steps. Quantitative differences could be reproducibly measured by flow cytometry at compound concentrations below the limit of detection for MS-based approaches. We establish the fluorogenic assay in E. coli and B. subtilis and compare the intracellular accumulation of two antibiotics, ciprofloxacin and ampicillin, with related pharmacophores in these bacteria.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu