Ferroptosis-protective membrane domains in quiescence.

Autor:	Megarioti AH; Microbial Molecular Genetics Laboratory, Institute of Biosciences and Applications, National Center for Scientific Research 'Demokritos,' 15341 Agia Paraskevi, Greece; Department of Biology, National and Kapodistrian University of Athens, Panepistimioupolis, 15784 Athens, Greece., Esch BM; Bioanalytical Chemistry Section, Department of Biology/Chemistry, Osnabrück University, 49076 Osnabrück, Germany., Athanasopoulos A; Microbial Molecular Genetics Laboratory, Institute of Biosciences and Applications, National Center for Scientific Research 'Demokritos,' 15341 Agia Paraskevi, Greece., Koulouris D; Microbial Molecular Genetics Laboratory, Institute of Biosciences and Applications, National Center for Scientific Research 'Demokritos,' 15341 Agia Paraskevi, Greece., Makridakis M; Biotechnology Division, Systems Biology Center, Biomedical Research Foundation of the Academy of Athens, 11527 Athens, Greece., Lygirou V; Biotechnology Division, Systems Biology Center, Biomedical Research Foundation of the Academy of Athens, 11527 Athens, Greece., Samiotaki M; Institute for Bioinnovation, Biomedical Sciences Research Center 'Alexander Fleming,' 16672 Vari, Greece., Zoidakis J; Department of Biology, National and Kapodistrian University of Athens, Panepistimioupolis, 15784 Athens, Greece; Biotechnology Division, Systems Biology Center, Biomedical Research Foundation of the Academy of Athens, 11527 Athens, Greece., Sophianopoulou V; Microbial Molecular Genetics Laboratory, Institute of Biosciences and Applications, National Center for Scientific Research 'Demokritos,' 15341 Agia Paraskevi, Greece., André B; Molecular Physiology of the Cell Laboratory, Université Libre de Bruxelles (ULB), IBMM, 6041 Gosselies, Belgium., Fröhlich F; Bioanalytical Chemistry Section, Department of Biology/Chemistry, Osnabrück University, 49076 Osnabrück, Germany; Center for Cellular Nanoanalytic Osnabrück (CellNanOs), Osnabrück University, 49076 Osnabrück, Germany. Electronic address: florian.froehlich@uni-osnabrueck.de., Gournas C; Microbial Molecular Genetics Laboratory, Institute of Biosciences and Applications, National Center for Scientific Research 'Demokritos,' 15341 Agia Paraskevi, Greece. Electronic address: cgournas@spfb.brussels.
Jazyk:	angličtina
Zdroj:	Cell reports [Cell Rep] 2023 Dec 26; Vol. 42 (12), pp. 113561. Date of Electronic Publication: 2023 Dec 12.
DOI:	10.1016/j.celrep.2023.113561
Abstrakt:	Quiescence is a common cellular state, required for stem cell maintenance and microorganismal survival under stress conditions or starvation. However, the mechanisms promoting quiescence maintenance remain poorly known. Plasma membrane components segregate into distinct microdomains, yet the role of this compartmentalization in quiescence remains unexplored. Here, we show that flavodoxin-like proteins (FLPs), ubiquinone reductases of the yeast eisosome membrane compartment, protect quiescent cells from lipid peroxidation and ferroptosis. Eisosomes and FLPs expand specifically in respiratory-active quiescent cells, and mutants lacking either show accelerated aging and defective quiescence maintenance and accumulate peroxidized phospholipids with monounsaturated or polyunsaturated fatty acids (PUFAs). FLPs are essential for the extramitochondrial regeneration of the lipophilic antioxidant ubiquinol. FLPs, alongside the Gpx1/2/3 glutathione peroxidases, prevent iron-driven, PUFA-dependent ferroptotic cell death. Our work describes ferroptosis-protective mechanisms in yeast and introduces plasma membrane compartmentalization as an important factor in the long-term survival of quiescent cells. Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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